A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element

被引：7
作者：
Vavrova, Aneta [1 ]
Vrzal, Radim [1 ]
Dvorak, Zdenek [1 ]
机构：
[1] Palacky Univ, Dept Cell Biol & Genet, Fac Sci, Olomouc 78371, Czech Republic
来源：
ELECTROPHORESIS | 2013年 / 34卷 / 13期
关键词：
Cytochrome P450; Drug metabolism; Electrophoretic mobility shift assay; Pregnane X receptor; Response elements; PROXIMAL PROMOTER; NUCLEAR RECEPTORS; GENE; PXR; IDENTIFICATION; CONSEQUENCES; HOMEOSTASIS; METABOLISM; ACTIVATION;
D O I：
10.1002/elps.201300079
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA-protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes and many other genes. Radiolabeled P-32-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences.
引用
收藏
页码：1863 / 1868
页数：6
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