Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration

被引：91

作者：

Francisco, Aubrey T. ^{[1
]}

Mancino, Robert J. ^{[1
]}

Bowles, Robby D. ^{[1
]}

Brunger, Jonathan M. ^{[1
,2
]}

Tainter, David M. ^{[3
]}

Chen, Yi-Te ^{[2
,4
,5
,6
]}

Richardson, William J. ^{[2
]}

Guilak, Farshid ^{[1
,2
]}

Setton, Lori A. ^{[1
,2
]}

机构：

[1] Duke Univ, Dept Biomed Engn, Durham, NC 27706 USA

[2] Duke Univ, Med Ctr, Dept Orthopaed Surg, Durham, NC USA

[3] Duke NUS Grad Med Sch, Singapore, Singapore

[4] Shin Kong Wu Ho Su Mem Hosp, Dept Orthoped, Taipei, Taiwan

[5] Fu Jen Catholic Univ, Sch Med, New Taipei City, Taiwan

[6] Natl Yang Ming Univ, Dept Orthoped, Taipei 112, Taiwan

来源：

BIOMATERIALS | 2013年 / 34卷 / 30期

关键词：

Laminin; Nucleus pulposus; Intervertebral disc; Polyethylene glycol; Injectable; Organ culture; MESENCHYMAL STEM-CELLS; INTERVERTEBRAL DISC CELLS; IN-VIVO; CHONDROCYTE TRANSPLANTATION; MATRIX INTERACTIONS; MODEL; DEGENERATION; EXPRESSION; THERAPY; REPAIR;

D O I：

10.1016/j.biomaterials.2013.06.038

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Cell delivery to the pathological intervertebral disc (IVD) has significant therapeutic potential for enhancing IVD regeneration. The development of injectable biomaterials that retain delivered cells, promote cell survival, and maintain or promote an NP cell phenotype in vivo remains a significant challenge. Previous studies have demonstrated NP cell - laminin interactions in the nucleus pulposus (NP) region of the IVD that promote cell attachment and biosynthesis. These findings suggest that incorporating laminin ligands into carriers for cell delivery may be beneficial for promoting NP cell survival and phenotype. Here, an injectable, laminin-111 functionalized poly(ethylene glycol) (PEG-LM111) hydrogel was developed as a biomaterial carrier for cell delivery to the IVD. We evaluated the mechanical properties of the PEG-LM111 hydrogel, and its ability to retain delivered cells in the IVD space. Gelation occurred in approximately 20 min without an initiator, with dynamic shear moduli in the range of 0.9-1.4 kPa. Primary NP cell retention in cultured IVD explants was significantly higher over 14 days when cells were delivered within a PEG-LM111 carrier, as compared to cells in liquid suspension. Together, these results suggest this injectable laminin-functionalized biomaterial may be an easy to use carrier for delivering cells to the IVD. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：7381 / 7388

页数：8

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