Value addition to corncob: Production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154

被引：127
作者：
Aachary, Ayyappan Appukuttan [1 ]
Prapulla, Siddalingaiya Gurudutt [1 ]
机构：
[1] Cent Food Technol Res Inst, Fermentat Technol & Bioengn Dept, Mysore 570020, Karnataka, India
来源：
BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 02期
关键词：
Xylooligosaccharides (XOS); Aspergillus oryzae MTCC 5154; Corncob; Lignin-saccharide complex; Endoxylanase; XYLO-OLIGOSACCHARIDES; THERMOTOGA-MARITIMA; XYLANASE-B; XYLOBIOSE; AUTOHYDROLYSIS; MANUFACTURE;
D O I：
10.1016/j.biortech.2008.06.050
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Comparison of various pretreatments such as mild alkali/acid treatments and pressure cooking of corncob to expose its lignin-saccharide complex has been carried out to enhance enzymatic hydrolysis of xylan to xylooligosaccharides (XOS). Scanning electron micrographs of lignin-saccharide complex of native and pretreated corncob powder showed that the complex was greatly altered during alkali pretreatment. Hydrolysis of alkali pretreated corncob powder using a commercial endoxylanase produced 81 +/- 1.5% of XOS in the hydrolyzate equivalent to 5.8 +/- 0.14 mg ml(-1) of XOS. Reaction parameters for the production of XOS from corncob using endoxylanase from Aspergillus oryzae MTCC 5154 were optimized and an XOS yield of 10.2 +/- 0.14 mg ml(-1) corresponding to 81 +/- 3.9% with 73.5% xylobiose was obtained. HPLC/RiD and ESI/MS analysis of XOS mixture and purified fractions showed that XOS was a mixture of neutral oligosaccharides of DP, 2-7. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：991 / 995
页数：5
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