Fluorescent detection of Hg2+ and Pb2+ using GeneFinder™ and an integrated functional nucleic acid

被引：41

作者：

Zhan, Shenshan ^{[1
,2
]}

Xu, Hanchu ^{[1
,2
]}

Zhang, Dongwei ^{[3
]}

Xia, Bing ^{[3
]}

Zhan, Xuejia ^{[1
,2
]}

Wang, Lumei ^{[1
,2
]}

Lv, Jing ^{[1
,2
]}

Zhou, Pei ^{[1
,2
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Agriculture & Biol, Sch Environm Sci & Engn, Key Lab Urban Agr South,Minist Agr, Shanghai 200240, Peoples R China

[2] Shanghai Jiao Tong Univ, Bar S Luh Food Safety Res Ctr, Shanghai 200240, Peoples R China

[3] Shanghai Inst Technol, Sch Chem & Environm Engn, Shanghai 201418, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 72卷

基金：

上海市自然科学基金; 中国国家自然科学基金;

关键词：

Fluorescent assay; Functional nucleic acid; Hg2+ detection; Pb2+ detection; T-Hg2+-T; G-quadruplex; LABEL-FREE; CHEMILUMINESCENCE SENSOR; ULTRASENSITIVE DETECTION; SELECTIVE DETECTION; QUADRUPLEX; MERCURY(II); IONS; DNA; LEAD(II); BIOSENSOR;

D O I：

10.1016/j.bios.2015.04.021

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This paper reports a simple fluorescent assay for the determination of Hg2+ and Pb2+ by using a DNA intercalator GeneFinder (TM) (GF) and an integrated functional nucleic acid (FNA). In the absence of Hg2+ and Pb2+, GF intercalated with the FNA and released moderate strong fluorescence. While in the presence of Hg2+ or Pb2+, the FNA would be induced to form T-Hg2+-T or G-quadruplex structure, interacted with which the GF would exhibit extremely strong or very weak fluorescence. By monitoring the fluorescence changes upon addition of these two ions, the Hg2+ and Pb2+ could be selectively detected as low as 3.23 ppb and 2.62 ppb. As the main advantage of this assay is simplicity and the feasibility was demonstrated by detecting Hg2+ and Pb2+ in spiked water samples, this assay holds great potential for the development of a cost effective and useful tool for environmental monitoring. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：95 / 99

页数：5

共 39 条

[1] "Turn-On" Chemiluminescence Sensor for the Highly Selective and Ultrasensitive Detection of Hg2+ Ions Based on Interstrand Cooperative Coordination and Catalytic Formation of Gold Nanoparticles
Cai, Sheng
Lao, Kameng
Lau, Choiwan
Lu, Jianzhong
[J]. ANALYTICAL CHEMISTRY, 2011, 83 (24) : 9702 - 9708
[2] Continuing Issues with Lead: Recent Advances in Detection
Deibler, Kristine
Basu, Partha
[J]. EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2013, (07) : 1086 - 1096
[3] Functional aptamers and aptazymes in biotechnology, diagnostics, and therapy
Famulok, Michael
Hartig, Jorg S.
Mayer, Gunter
[J]. CHEMICAL REVIEWS, 2007, 107 (09) : 3715 - 3743
[4] A label-free thrombin binding aptamer as a probe for highly sensitive and selective detection of lead(II) ions by a resonance Rayleigh scattering method
Fu, Xiao Bei
Qu, Fei
Li, Nian Bing
Luo, Hong Qun
[J]. ANALYST, 2012, 137 (05) : 1097 - 1099
[5] The biological chemistry of lead
Godwin, HA
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (02) : 223 - 227
[6] A G-quadruplex based label-free fluorescent biosensor for lead ion
Guo, Liangqia
Nie, Dandan
Qiu, Chunyan
Zheng, Qishan
Wu, Haiyan
Ye, Peirong
Hao, Yanli
Fu, FengFu
Chen, Guonan
[J]. BIOSENSORS & BIOELECTRONICS, 2012, 35 (01) : 123 - 127
[7] An ultrasensitive electrochemical sensor for the mercuric ion via controlled assembly of SWCNTs
Guo, Liangqia
Yin, Na
Nie, Dandan
Fu, Fengfu
Chen, Guonan
[J]. CHEMICAL COMMUNICATIONS, 2011, 47 (38) : 10665 - 10667
[8] Selective gold-nanoparticle-based "turn-on" fluorescent sensors for detection of mercury(II) in aqueous solution
Huang, Chih-Ching
Chang, Huan-Tsung
[J]. ANALYTICAL CHEMISTRY, 2006, 78 (24) : 8332 - 8338
[9] Methods for the determination and speciation of mercury in natural waters-A review
Leopold, Kerstin
Foulkes, Michael
Worsfold, Paul
[J]. ANALYTICA CHIMICA ACTA, 2010, 663 (02) : 127 - 138
[10] Li D., 2008, ANGEW CHEM, P3991

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