High Mineralization Capacity of IDG-SW3 Cells in 3D Collagen Hydrogel for Bone Healing in Estrogen-Deficient Mice

被引：7

作者：

Chen, Kaizhe ^{[1
]}

Zhou, Qi ^{[1
]}

Kang, Hui ^{[2
]}

Yan, Yufei ^{[3
]}

Qian, Niandong ^{[1
]}

Li, Changwei ^{[1
]}

Wang, Fei ^{[1
]}

Yang, Kai ^{[1
]}

Deng, Lianfu ^{[1
]}

Qi, Jin ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Shanghai Key Lab Prevent & Treatment Bone & Joint, Shanghai Inst Traumatol & Orthopaed,Ruijin Hosp, Shanghai, Peoples R China

[2] Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Dept Orthoped, Shanghai, Peoples R China

[3] Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6, Dept Orthoped Surg, Shanghai, Peoples R China

来源：

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2020年 / 8卷

基金：

中国国家自然科学基金;

关键词：

tissue regeneration; BMSCs; osteoblast; bone; osteocyte; MESENCHYMAL STEM-CELLS; IN-VITRO; OSTEOCYTES; RECONSTRUCTION; HOMEOSTASIS; CLEAVAGE; DEFECTS;

D O I：

10.3389/fbioe.2020.00864

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tissue engineering with 3D scaffold is a simple and effective method for bone healing after large-scale bone loss. So far, bone marrow-derived mesenchymal stem cells (BMSCs) are mostly used in the treatment of bone healing in animal models due to their self-renewal capability and osteogenic potential. Due to the fact that the main functional cells in promoting osteoid mineralization and bone remodeling were osteocytes, we chose an osteoblast-to-osteocyte transition cell line, IDG-SW3, which are not proliferative under physiological conditions, and compared the healing capability of these cells to that of BMSCs in bone defect.In vitro, IDG-SW3 cells revealed a stronger mineralization capacity when grown in 3D collagen gel, compared to that of BMSCs. Although both BMSC and IDG-SW3 can generate stable calcium-phosphate crystal similar to hydroxyapatite (HA), the content was much more enriched in IDG-SW3-mixed collagen gel. Moreover, the osteoclasts co-cultured with IDG-SW3-mixed collagen gel were easier to be activated, indicating that the IDG-SW3 grafting could promote the bone remodeling more efficientlyin vivo. Last, in order to reduce the self-healing capability, we assessed the healing capability between the IDG-SW3 cells and BMSCs in osteoporotic mice. We found that the collagen hydrogel mixed with IDG-SW3 cells has a better healing pattern than what was seen in hydrogel mixed with BMSCs. Therefore, these results demonstrated that by promoting osteoblast-to-osteocyte transition, the therapeutic effect of BMSCs in bone defect repair could be improved.

引用

页数：11

共 30 条

[1] Mechanism by which MLO-A5 late osteoblasts/early osteocytes mineralize in culture: Similarities with mineralization of lamellar bone [J].