Transforming Growth Factor Beta 3 Is Required for Excisional Wound Repair In Vivo

被引：70
作者：
Le, Mark [1 ]
Naridze, Rachelle [1 ]
Morrison, Jasmine [1 ]
Biggs, Leah C. [1 ]
Rhea, Lindsey [1 ]
Schutte, Brian C. [2 ]
Kaartinen, Vesa [3 ]
Dunnwald, Martine [1 ]
机构：
[1] Univ Iowa, Dept Pediat, Carver Coll Med, Iowa City, IA 52242 USA
[2] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA
[3] Univ Michigan, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA
来源：
PLOS ONE | 2012年 / 7卷 / 10期
基金：
美国国家卫生研究院;
关键词：
SCAR TISSUE FORMATION; TGF-BETA; EMBRYONIC-DEVELOPMENT; EXPRESSION PATTERNS; KNOCKOUT MICE; CLEFT-PALATE; P38; MAPK; TGF-BETA-3; SKIN; MOUSE;
D O I：
10.1371/journal.pone.0048040
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Wound healing is a complex process that relies on proper levels of cytokines and growth factors to successfully repair the tissue. Of particular interest are the members of the transforming growth factor family. There are three TGF-beta isoforms-TGF beta 1, 2, and 3, each isoform showing a unique expression pattern, suggesting that they each play a distinct function during development and repair. Previous studies reported an exclusive role for TGF-beta 3 in orofacial development and a potent anti-scarring effect. However, the role of TGF-beta 3 in excisional wound healing and keratinocyte migration remains poorly understood. We tested the effect of TGF-beta 3 levels on excisional cutaneous wounds in the adult mouse by directly injecting recombinant TGF-beta 3 or neutralizing antibody against TGF-beta 3 (NAB) in the wounds. Our results demonstrate that TGF-beta 3 does not promote epithelialization. However, TGF-beta 3 is necessary for wound closure as wounds injected with neutralizing antibody against TGF-beta 3 showed increased epidermal volume and proliferation in conjunction with a delay in keratinocyte migration. Wild type keratinocytes treated with NAB and Tgfb3-deficient keratinocytes closed an in vitro scratch wound with no delay, suggesting that our in vivo observations likely result from a paracrine effect.
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页数：10
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