Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato

被引：6
作者：
Wang Zi-yu [1 ]
Bao Yu-fang [1 ]
Pei Tong [1 ]
Wu Tai-ru [1 ]
Du Xu [1 ]
He Meng-xi [1 ]
Wang Yue [1 ]
Liu Qi-feng [1 ]
Yang Huan-huan [1 ]
Jiang Jing-bin [1 ]
Zhang He [1 ]
Li Jing-fu [1 ]
Zhao Ting-ting [1 ]
Xu Xiang-yang [1 ,2 ]
机构：
[1] Northeast Agr Univ, Coll Hort & Landscape Architecture, Harbin 150030, Peoples R China
[2] Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Hort Crops North, Harbin 150030, Peoples R China
来源：
JOURNAL OF INTEGRATIVE AGRICULTURE | 2020年 / 19卷 / 11期
基金：
国家重点研发计划; 中国博士后科学基金;
关键词：
SLB3; BES1 transcription factor; VIGS; drought; gene expression; BR signaling pathway; GENOME-WIDE IDENTIFICATION; ABIOTIC STRESS; INDUCED EXPRESSION; ARABIDOPSIS TCH4; RESPONSES; ROLES; BES1; BZR1; HOMEOSTASIS; NETWORK;
D O I：
10.1016/S2095-3119(20)63350-0
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
BRI1-EMS-SUPPRESSOR 1 (BES1)transcription factor is closely associated with the brassinosteroid (BR) signaling pathway and plays an important role in plant growth and development. SLB3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought stress. In the present study, we used virus-induced gene silencing (VIGS) technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress further. The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H2O2 and O-2(-center dot), decreased superoxide dismutase (SOD) activity, and increased proline (PRO) and malondialdehyde (MDA) contents and peroxidase (POD) activity. Quantitative real-time PCR (qRT-PCR) analysis of BR-related genes revealed that the expression of SICPD, SIDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress, but that the expression of TCH4-related genes was downregulated. These results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants.
引用
收藏
页码：2699 / 2708
页数：10
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