Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through Gβγ-Mediated Pathway

被引：8

作者：

Shin, Min-Hee ^{[1
]}

Choi, Eun-Kyeong ^{[1
]}

Kim, Ki-Suk ^{[1
]}

Kim, Kang-Hoon ^{[1
]}

Jang, Young Pyo ^{[2
]}

Ahn, Kwang Seok ^{[1
]}

Chung, Won-Seok ^{[1
]}

Cha, Nam Hyun ^{[3
]}

Jang, Hyeung-Jin ^{[1
]}

机构：

[1] Kyung Hee Univ, Inst Korean Med, Coll Korean Med, Seoul 130701, South Korea

[2] Kyung Hee Univ, Coll Pharm, Seoul 130701, South Korea

[3] Kangwon Univ, Coll Hlth Sci, Dept Nursing, Chuncheon Si 200701, South Korea

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2014年 / 2014卷

基金：

新加坡国家研究基金会;

关键词：

BITTER TASTE RECEPTORS; MEMBRANE DEPOLARIZATION; TRANSCRIPTOMIC ANALYSIS; PHOSPHOLIPASE-C; CALCIUM; EXTRACTS; GLP-1;

D O I：

10.1155/2014/982165

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Bupleurum falcatum L. has been used traditionally as a medicinal herb in Korean medicine. The hexane fraction of BF (HFBF), which was profiled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS), activates the secretion of glucagon-like peptide-1 (GLP-1) in NCI-H716 cells significantly. We performed a microarray analysis and GLP-1 ELISA assay, as well as calcium imaging experiments with inhibitors, to investigate the mechanism of action of the HFBF. Through the microarray analysis, it was found that the ITPR2 gene that encodes the inositol 1,4,5-trisphosphate (IP3) receptor is up-regulated and the HFBF induces cell depolarization by inhibiting the voltage-gated channel expression in NCI-H716 cells. In addition, we found that the intracellular calcium in NCI-H716 cells, with Gallein, U73122, and 2APB as inhibitors, was decreased. These results suggest that the HFBF activates the GLP-1 secretion through the G(beta gamma) pathways in the enteroendocrine L cells after treatment with the HFBF.

引用

页数：8

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