Combination treatment with platycodin D and osthole inhibits cell proliferation and invasion in mammary carcinoma cell lines

被引:45
作者
Ye, Yiyi [1 ]
Han, Xianghui [1 ]
Guo, Baofeng [1 ]
Sun, Zhenping [2 ]
Liu, Sheng [1 ,2 ]
机构
[1] Longhua Hosp, Pharmacol Lab Tradit Chinese Med, Shanghai 200032, Peoples R China
[2] Longhua Hosp, Dept Breast Surg, Shanghai 200032, Peoples R China
基金
中国国家自然科学基金;
关键词
Platycodin D; Osthole; Combination treatment; Murine; 4T1; cells; Human MDA-MB-231 cells; Anti-invasion; TGF-BETA; MESENCHYMAL TRANSITION; BONE METASTASIS; CANCER-CELLS; KAPPA-B; TUMOR; EXPRESSION; SPECIFICITY; APOPTOSIS; PATHWAY;
D O I
10.1016/j.etap.2013.03.012
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
In this study, two invasive mammary carcinoma cells (MDA-MB-231 and 4T1) were utilized to evaluate the inhibitory activities of platycodin D, osthole, and the two in combination. The anti-proliferative effect was tested using the MTT and BrdU assay, and the combination of 15 mu M osthole and 75 mu M platycodin D was used for subsequent analyses. The anti-invasive effect was evaluated by the transwell assay. The results showed that the combination treatment reduced both cell proliferation and invasion. Western blot and real-time PCR revealed that the platycodin D-osthole combination significantly decreased T beta RII, Smad2, Smad3 and Smad4 gene or protein expressions, as well as effectively blocked TGF-beta-induced phosphorylation of Smad2 and Smad3. Thus, this study demonstrates that the anti-cancer effects of the platycodin D-osthole combination in breast cancer cells involve proliferation inhibition and invasion blockade, both of which maybe mediated by perturbations in the TGF-beta/Smads pathway. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:115 / 124
页数:10
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