Virus-specific capping of tobacco mosaic virus RNA:: methylation of GTP prior to formation of covalent complex p126-m7GMP

被引：46

作者：

Merits, A ^{[1
]}

Kettunen, R ^{[1
]}

Mäkinen, K ^{[1
]}

Lampio, A ^{[1
]}

Auvinen, P ^{[1
]}

Kääriäinen, L ^{[1
]}

Ahola, T ^{[1
]}

机构：

[1] Univ Helsinki, Bioctr Viikki, Inst Biotechnol, FIN-00014 Helsinki, Finland

来源：

FEBS LETTERS | 1999年 / 455卷 / 1-2期

基金：

芬兰科学院;

关键词：

plant virus; RNA replication; guanylyltransferase; methyltransferase; tobacco mosaic virus;

D O I：

10.1016/S0014-5793(99)00856-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In capping cellular mRNAs, a covalent GMP-enzyme intermediate leads to formation of G(S')ppp(5')N at time 5' end of the RNA, which is modified by methylation catalyzed by guanine-7-methyltransferase. Here we show that isolated membranes from tobacco mosaic virus (TMV)-infected plant or insect cells expressing TMV replicase protein p126, synthesized m(7) GTP using S-adenosylmethionine (AdoMet) as the methyl donor, and catalyzed the formation of a covalent guanylate-p126 complex in the presence of AdoMet, The methyl group from AdoMet was incorporated into p126, suggesting that the complex consisted of m(7)GMP-p126, Thus, TMV and alphaviruses, despite their evolutionary distance, share the same virus-specific capping mechanism. (C) 1999 Federation of European Biochemical Societies.

引用

页码：45 / 48

页数：4

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