Preparative separation of isomeric caffeoylquinic acids from Flos Lonicerae by pH-zone-refining counter-current chromatography

被引：34

作者：

Tong, Shengqiang ^{[1
,2
]}

Yan, Jizhong ^{[1
,3
]}

Guan, Yi-Xin ^{[2
]}

机构：

[1] Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou 310032, Zhejiang, Peoples R China

[2] Zhejiang Univ, Dept Chem & Biochem Engn, Hangzhou 310027, Zhejiang, Peoples R China

[3] Zhejiang Univ, Pharmaceut Informat Inst, Coll Pharmaceut Sci, Hangzhou 310027, Zhejiang, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1212卷 / 1-2期

关键词：

pH-zone-refining counter-current chromatography; Preparative chromatography; Caffeoylquinic acids; Separation;

D O I：

10.1016/j.chroma.2008.09.114

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This work concentrates on pH-zone-refining counter-current chromatography of two isomeric dicaffeoylquinic acids, 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid along with 3-caffeoylquinic acid, from crude extracts of Flos Lonicerae. The elution sequence of the isomeric dicaffeoylquinic acids, the mixing zone and mechanism of separation are discussed. The separation of 2.136g of the crude sample from Flos Lonicerae yielded two isomeric compounds: 0.289g 3,5-dicaffeoylquinic acid and 0.106g 3,4-dicaffeoylquinic acid plus 0.690g 3-caffeoylquinic acid at a high purity of over 92.9%, 94.2% and 97.5%, respectively. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：48 / 53

页数：6

共 48 条

[11] Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography [J].

Ito, Y .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1065 (02) :145-168

[12]

ITO Y, 1995, ACS SYM SER, V593, P156

[13]

Ito Y., 1996, HIGH SPEED COUNTER C

[14]

ITO Y, 1994, Patent No. 5332404

[15] Determination of five maior iridoid glucosides in Flos Lonicerae by high-performance liquid chromatography coupled with evaporative light scattering detection [J].

Li, HJ ;

Li, P ;

Ye, WC .

JOURNAL OF CHROMATOGRAPHY A, 2003, 1008 (02) :167-172

[16]

Li Yong-mei, 2001, Zhongguo Zhongyao Zazhi, V26, P45

[17] Application of preparative high-speed counter-current chromatography for separation of chlorogenic acid from Flos Lonicerae [J].

Lu, HT ;

Jiang, Y ;

Chen, F .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1026 (1-2) :185-190

[18] pH-zone refining counter-current chromatography of polar catecholamines using di-(2-ethylhexyl)phosphoric acid as a ligand [J].

Ma, Y ;

Sokoloski, E ;

Ito, Y .

JOURNAL OF CHROMATOGRAPHY A, 1996, 724 (1-2) :348-353

[19] RESOLUTION OF GRAM QUANTITIES OF RACEMATES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY [J].

MA, Y ;

ITO, Y ;

FOUCAULT, A .

JOURNAL OF CHROMATOGRAPHY A, 1995, 704 (01) :75-81

[20] SEPARATION OF THE 2-NITRO-3-ACETAMIDO-4-CHLOROBENZOIC AND 6-NITRO-3-ACETAMIDO-4-CHLOROBENZOIC ACID PRECURSORS OF A POTENT HYDROXY-ANTHRANILIC ACID OXYGENASE INHIBITOR BY PH-ZONE-REFINING-COUNTERCURRENT CHROMATOGRAPHY [J].

MA, Y ;

ITO, Y ;

TOROK, DS ;

ZIFFER, H .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1994, 17 (16) :3507-3517

← 1 2 3 4 5 →