Preparative separation of isomeric caffeoylquinic acids from Flos Lonicerae by pH-zone-refining counter-current chromatography

被引：34

作者：

Tong, Shengqiang ^{[1
,2
]}

Yan, Jizhong ^{[1
,3
]}

Guan, Yi-Xin ^{[2
]}

机构：

[1] Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou 310032, Zhejiang, Peoples R China

[2] Zhejiang Univ, Dept Chem & Biochem Engn, Hangzhou 310027, Zhejiang, Peoples R China

[3] Zhejiang Univ, Pharmaceut Informat Inst, Coll Pharmaceut Sci, Hangzhou 310027, Zhejiang, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1212卷 / 1-2期

关键词：

pH-zone-refining counter-current chromatography; Preparative chromatography; Caffeoylquinic acids; Separation;

D O I：

10.1016/j.chroma.2008.09.114

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This work concentrates on pH-zone-refining counter-current chromatography of two isomeric dicaffeoylquinic acids, 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid along with 3-caffeoylquinic acid, from crude extracts of Flos Lonicerae. The elution sequence of the isomeric dicaffeoylquinic acids, the mixing zone and mechanism of separation are discussed. The separation of 2.136g of the crude sample from Flos Lonicerae yielded two isomeric compounds: 0.289g 3,5-dicaffeoylquinic acid and 0.106g 3,4-dicaffeoylquinic acid plus 0.690g 3-caffeoylquinic acid at a high purity of over 92.9%, 94.2% and 97.5%, respectively. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：48 / 53

页数：6

共 48 条

[1]

BILLARDELLO B, 1994, COMPREHENSIVE ANAL C, V38, P181

[2] Quality evaluation of Flos Lonicerae through a simultaneous determination of seven saponins by HPLC with ELSD [J].

Chai, XY ;

Li, SL ;

Li, P .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1070 (1-2) :43-48

[3] Capillary high-performance liquid chromatography with mass spectrometry for simultaneous determination of major flavonoids, iridoid glucosides and saponins in Flos Lonicerae [J].

Chen, Jun ;

Song, Yue ;

Li, Ping .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1157 (1-2) :217-226

[4]

*CHIN PHARM COMM, 2000, PHARM PEOPL REP CHIN, P177

[5] PREPARATIVE SEPARATION OF STEREOISOMERIC 1-METHYL-4-METHOXYMETHYLCYCLOHEXANECARBOXYLIC ACIDS BY PH-ZONE-REFINING COUNTERCURRENT CHROMATOGRAPHY [J].

DENEKAMP, C ;

MANDELBAUM, A ;

WEISZ, A ;

ITO, Y .

JOURNAL OF CHROMATOGRAPHY A, 1994, 685 (02) :253-257

[6] Use of pH-zone-refining countercurrent chromatography to separate 2- and 6-nitro-4-chloro-3-methoxybenzoic acid [J].

Dudding, T ;

Mekonnen, B ;

Ito, Y ;

Ziffer, H .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1998, 21 (1-2) :195-201

[7] Enantiomer separation by countercurrent chromatography using cinchona alkaloid derivatives as chiral selectors [J].

Franco, P ;

Blanc, J ;

Oberleitner, WR ;

Maier, NM ;

Lindner, W ;

Minguillón, C .

ANALYTICAL CHEMISTRY, 2002, 74 (16) :4175-4183

[8] Rational development of solvent system families in counter-current chromatography [J].

Friesen, J. Brent ;

Pauli, Guido F. .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1151 (1-2) :51-59

[9]

GOU ZP, 2006, ZHONGCHENGYAO, V28, P408

[10] pH-zone-refining countercurrent chromatography [J].

Ito, Y ;

Ma, Y .

JOURNAL OF CHROMATOGRAPHY A, 1996, 753 (01) :1-36

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