Gene therapy of chondrosarcoma using retrovirus vectors encoding the herpes simplex virus thymidine kinase gene

被引：0
|
作者：
Seto, M
Wakabayashi, H
Yamazaki, T
Sonoda, J
Shinto, Y
Uchida, A
机构：
[1] Mie Univ, Sch Med, Dept Orthopaed Surg, Tsu, Mie 5148507, Japan
[2] Osaka Hosp Railrd Employees, Dept Orthopaed Surg, Osaka, Japan
来源：
INTERNATIONAL JOURNAL OF ONCOLOGY | 1999年 / 14卷 / 06期
关键词：
chondrosarcoma; gene therapy; retrovirus vectors; suicide gene; bystander effect;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Human chondrosarcoma cells (HCS-TG) were transduced with the gene for a herpes simplex virus thymidine kinase (HSV-tk) or Escherichia coli beta-galactosidase (lacZ). We investigated the cytotoxicity of human chondrosarcoma bearing an HSV-tk gene after treatment with ganciclovir. Chondrosarcoma cells bearing an HSV-tk gene were more sensitive than non-transduced cells. Coculturing with chondrosarcoma cells bearing both an HSV-tk gene (HCS-TG-tk) and lacZ gene (HCS-TG-Z) in various ratios showed a bystander effect. Chondrosarcoma implanted in nude mice were injected with HCS-TG-tk cells. After 4 weeks, the growth of tumors was significantly prevented.
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页码：1137 / 1141
页数：5
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