Probing chiral amino acids at sub-picomolar level based on bovine serum albumin enantio selective films coupled with silver-enhanced gold nanoparticles

被引：63

作者：

Wang, Yunxia ^{[1
]}

Yin, Xiuli ^{[1
]}

Shi, Mianhong ^{[1
]}

Li, Wei ^{[1
]}

Zhang, Lei ^{[1
]}

Kong, Jilie ^{[1
]}

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

来源：

TALANTA | 2006年 / 69卷 / 05期

基金：

中国国家自然科学基金;

关键词：

electrochemical sensor; chiral recognition; bovine serum albumin (BSA); chiral amino acids; gold nanoparticles; silver enhancement;

D O I：

10.1016/j.talanta.2005.12.060

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel electrochemical sensor with capability of probing chiral amino acids with gold nanoparticle (n-Au) labels using bovine serum albumin (BSA) as a chiral selector and subsequent signal amplification step by silver enhancement is introduced. The assay relies on the stereoselectivity of BSA embedded in ultrathin gamma-alumina sol-gel film coated on the surface of the glassy carbon electrode (GCE). The recognition to the n-Au-labeled L- or D-amino acids for BSA-GCE could be monitored by the differential pulse voltammetry (DPV), while the DPV signal was greatly amplified by the anchored silver atoms on the n-Au, leading to a new way of quantitatively analysis of chiral amino acids electrochemically at sub-picomolar level. With L-tryptophan as the probe solute, the linear concentration range was from 1.33 x 10(-12) to 1 x 10(-9) mol L-1 and detection limit was 5 x 10(-13) mol L-1. For tryptophan enantiomers, the enantioselectivity coefficient 2.3 was obtained. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1240 / 1245

页数：6