Clinical Significance of Long Intergenic Noncoding RNA-p21 in Colorectal Cancer

被引：126
作者：
Zhai, Haiyan [1 ]
Fesler, Andrew [1 ]
Schee, Kristina [2 ]
Fodstad, Oystein [2 ]
Flatmark, Kjersti [2 ,3 ]
Ju, Jingfang [1 ]
机构：
[1] SUNY Stony Brook, Dept Pathol, Stony Brook, NY 11794 USA
[2] Oslo Univ Hosp, Norwegian Radium Hosp, Dept Tumor Biol, Oslo, Norway
[3] Oslo Univ Hosp, Norwegian Radium Hosp, Dept Surg Gastroenterol, Oslo, Norway
来源：
CLINICAL COLORECTAL CANCER | 2013年 / 12卷 / 04期
关键词：
Colorectal cancer; lincRNA-p21; p53; BETA-CATENIN EXPRESSION; COLON-CANCER; DIFFERENTIAL EXPRESSION; PROGNOSTIC-SIGNIFICANCE; RNA HOTAIR; P53; CELLS; CHEMORESISTANCE; OSTEOSARCOMA; ACCUMULATION;
D O I：
10.1016/j.clcc.2013.06.003
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The impact of long intergenic noncoding RNA (lincRNA)-p21 on colorectal cancer (CRC) progression was investigated in this study. Wild-type p53 induces endogenous lincRNA-p21 in HCT-116 colon cancer cells. Elevated levels of lincRNA-p21 were significantly associated with CRC disease state, pT, and vascular invasion, indicating that lincRNA-p21 may contribute to CRC progression. Background: Long intergenic noncoding RNAs (lincRNAs) have been shown to be novel regulators for both transcription and posttranscriptional/translation. One of them, lincRNA-p21, was regulated by p53 and contributed to apoptosis in mouse embryonic fibroblasts. However, the impact of such regulation on colorectal cancer (CRC) remains to be determined. Methods: Total RNA was extracted from CRC cell lines and snap fresh frozen CRC samples from 2 CRC patient cohorts. The expression of lincRNA-p21 was quantified by quantitative real-time polymerase chain reaction analysis. Results: We discovered that the expression level of lincRNA-p21 was increased by elevated wild-type p53 induced by nutlin-3 in HCT-116 colon cancer cells. The expression level of lincRNA-p21 was significantly (P = .0208) lower in CRC tumor tissue when compared with the paired normal tissue from the same patient. There was no significant correlation of lincRNA-p21 with p53 status (wild-type vs. mutant). Tumors in the rectum showed a higher level of lincRNA-p21 than tumors in the colon (P = .00005). In addition, lincRNA-p21 in patients with stage III tumors was significantly higher than in those with stage I tumors (P = .007). Elevated levels of lincRNA-p21 were significantly associated with higher pT (P = .037 between pT 2 and 3) and vascular invasion (P = .017). Conclusions: These results indicate that lincRNA-p21 may contribute to CRC disease progression.
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收藏
页码：261 / 266
页数：6
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