Enhanced acetyl esterase production by Fusarium oxysporum

被引：13

作者：

Christakopoulos, P ^{[1
]}

Mamma, D ^{[1
]}

Kekos, D ^{[1
]}

Macris, BJ ^{[1
]}

机构：

[1] Natl Tech Univ Athens, Dept Chem Engn, GR-15780 Athens, Greece

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1999年 / 15卷 / 04期

关键词：

acetyl (xylan) esterase production; Fusarium oxysporum;

D O I：

10.1023/A:1008936204368

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Production of acetyl esterase (EC 3.1.1.6) by Fusarium oxysporum strain F3 was enhanced by optimization of growth conditions. Under optimal conditions, activities as high as 0.89 U/ml of culture medium were obtained. The culture filtrate was equally active on p-nitrophenyl acetate and acetylxylan. The enzyme produced 71% deacetylation of acetylxylan in 2 h at 40 degrees C. Activity was optimized at pH 6.5 and at 55 degrees C. The respective K-m values for p-nitrophenyl acetate and acetylxylan were 0.25 mM and 1.05% (w/v) and the V-m values were 0.65 and 0.43 mu mol acetate/min/mg protein.

引用

页码：443 / 446

页数：4

共 15 条

[1] PURIFICATION AND COOPERATIVE ACTIVITY OF ENZYMES CONSTITUTING THE XYLAN-DEGRADING SYSTEM OF THERMOMONOSPORA-FUSCA [J].

BACHMANN, SL ;

MCCARTHY, AJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (08) :2121-2130

[2]

BIELY P, 1988, CAN J MICROBIOL, V34, P767, DOI 10.1139/m88-130

[3] Substrate specificity of acetylxylan esterase from Schizophyllum commune: Mode of action on acetylated carbohydrates [J].

Biely, P ;

Cote, GL ;

Kremnicky, L ;

Weisleder, D ;

Greene, RV .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1996, 1298 (02) :209-222

[4] N-(1-NAPHTHYL)ETHYLENEDIAMINE DIHYDROCHLORIDE AS A NEW REAGENT FOR NANOMOLE QUANTIFICATION OF SUGARS ON THIN-LAYER PLATES BY A MATHEMATICAL CALIBRATION PROCESS [J].

BOUNIAS, M .

ANALYTICAL BIOCHEMISTRY, 1980, 106 (02) :291-295

[5] DIRECT FERMENTATION OF CELLULOSE TO ETHANOL BY FUSARIUM-OXYSPORUM [J].

CHRISTAKOPOULOS, P ;

MACRIS, BJ ;

KEKOS, D .

ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (04) :236-239

[6] Production and partial characterization of xylanase from Fusarium oxysporum [J].

Christakopoulos, P ;

Mamma, D ;

Nerinckx, W ;

Kekos, D ;

Macris, B ;

Claeyssens, M .

BIORESOURCE TECHNOLOGY, 1996, 58 (02) :115-119

[7] CONTROLLING SIMULTANEOUS PRODUCTION OF ENDOGLUCANASE AND BETA-GLUCOSIDASE BY FUSARIUM-OXYSPORUM IN SUBMERGED CULTURE [J].

CHRISTAKOPOULOS, P ;

KEKOS, D ;

KOLISIS, FN ;

MACRIS, BJ .

BIOTECHNOLOGY LETTERS, 1995, 17 (08) :883-888

[8] ESTERASES OF XYLAN-DEGRADING MICROORGANISMS - PRODUCTION, PROPERTIES, AND SIGNIFICANCE [J].

CHRISTOV, LP ;

PRIOR, BA .

ENZYME AND MICROBIAL TECHNOLOGY, 1993, 15 (06) :460-475

[9] THE ROLE OF ESTER GROUPS IN RESISTANCE OF PLANT-CELL WALL POLYSACCHARIDES TO ENZYMATIC-HYDROLYSIS [J].

GROHMANN, K ;

MITCHELL, DJ ;

HIMMEL, ME ;

DALE, BE ;

SCHROEDER, HA .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1989, 20-1 :45-61

[10]

Kennedy J.F., 1974, ADV CARBOHYD CHEM BI, V29, P305

← 1 2 →