Through the eye of an electrospray needle: mass spectrometric identification of the major peptides and proteins in the milk of the one-humped camel (Camelus dromedarius)

被引:25
作者
Alhaider, Abdulqader [1 ]
Abdelgader, Abdel Galil [2 ]
Turjoman, Abdullah Arif [3 ,4 ]
Newell, Keri
Hunsucker, Stephen W. [5 ]
Shan, Baozhen [7 ]
Ma, Bin [8 ]
Gibson, David S. [9 ]
Duncan, Mark W. [3 ,6 ]
机构
[1] King Saud Univ, Dept Pharmacol, Riyadh, Saudi Arabia
[2] King Saud Univ, Dept Physiol, Riyadh, Saudi Arabia
[3] King Saud Univ, Obes Res Ctr, Riyadh, Saudi Arabia
[4] King Saud Univ, Coll Med, Dept Pathol, Clin Chem Unit, Riyadh 11461, Saudi Arabia
[5] Univ Colorado Denver, Sect Pulm Med, Dept Pediat, Aurora, CO 80045 USA
[6] Univ Colorado Denver, Sch Med, Aurora, CO 80045 USA
[7] Bioinformat Solut Inc, Waterloo, ON, Canada
[8] Univ Waterloo, Sch Comp Sci, Waterloo, ON N2L 3G1, Canada
[9] Queens Univ Belfast, Arthrit Res Grp, Ctr Infect & Immun, Belfast BT9 7BL, Antrim, North Ireland
来源
JOURNAL OF MASS SPECTROMETRY | 2013年 / 48卷 / 07期
关键词
DIGE; mass spectrometry; proteomics; proteins; peptides; camel milk; Camelus dromedarius; C VIRUS-INFECTION; BIOACTIVE PEPTIDES; PASSIVE-IMMUNITY; BIOLOGICAL SAMPLES; BLOOD-PRESSURE; BOVINE; FOOD; ANTIBODIES; LACTOFERRIN; CASEIN;
D O I
10.1002/jms.3213
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The milk of the one-humped camel (Camelus dromedarius) reportedly offers medicinal benefits, perhaps because of its unique bioactive components. Milk proteins were determined by (1) two-dimensional gel electrophoresis and peptide mass mapping and (2) liquid chromatography-tandem mass spectrometry (LC-MS/MS) following one-dimensional polyacrylamide gel electrophoresis. Over 200 proteins were identified: some known camel proteins including heavy-chain immunoglobulins and others exhibiting regions of exact homology with proteins from other species. Indigenous peptides were also identified following isolation and concentration by two strategies: (1) gel-eluted liquid fraction entrapment electrophoresis and (2) small-scale electrophoretic separation. Extracts were analyzed by LC-MS/MS and peptides identified by matching strategies, by de novo sequencing and by applying a sequence tag tool requiring similarity to the proposed sequence, but not an exact match. A plethora of protein cleavage products including some novel peptides were characterized. These studies demonstrate that camel milk is a rich source of peptides, some of which may serve as nutraceuticals. Copyright (c) 2013 John Wiley & Sons, Ltd.
引用
收藏
页码:779 / 794
页数:16
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