GLYCOLIPID CONTENT OF THE NUCLEAR MEMBRANE IN HYPOXIC RAT BRAIN

被引:0
作者
Petrova, E. [1 ]
Dishkelov, A. [1 ]
Vasileva, E. [1 ]
Gramatikova, T. [1 ]
Ormandzhieva, V. [1 ]
Dimitrova, S. [2 ]
机构
[1] Bulgarian Acad Sci, Inst Expt Morphol Pathol & Anthropol Museum, Sofia, Bulgaria
[2] Bulgarian Acad Sci, Space & Solar Terr Res Inst, Sofia, Bulgaria
关键词
glycolipids; hypoxia; sodium nitrite; nuclear subcellular fraction; rat brain;
D O I
10.5504/50YRTIMB.2011.0014
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this study, we report glycolipid changes in the nuclear subcellular fraction from hypoxic rat brains. Male Wistar rats at the age of three months were subjected to sodium nitrite-induced hypoxia. Te nuclear fraction was isolated and lipids were extracted. The glycolipid content was measured by spectrophotometry and thin-layer chromatography. In controls, gangliosides and cerebrosides were the major glycolipid classes. In the nuclear membrane of hypoxic brains, we found increased levels of total glycolipids (5.2-fold), gangliosides (4.1-fold), and cerebrosides (5.9-fold). These changes reflect the disturbances in the glycolipid turnover. Most probably they are associated with impaired energy metabolism. The accumulation of glycolipids may be interpreted as a physiological adaptive response to hypoxia.
引用
收藏
页码:77 / 79
页数:3
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