GLYCOLIPID CONTENT OF THE NUCLEAR MEMBRANE IN HYPOXIC RAT BRAIN

被引：0

作者：

Petrova, E. ^{[1
]}

Dishkelov, A. ^{[1
]}

Vasileva, E. ^{[1
]}

Gramatikova, T. ^{[1
]}

Ormandzhieva, V. ^{[1
]}

Dimitrova, S. ^{[2
]}

机构：

[1] Bulgarian Acad Sci, Inst Expt Morphol Pathol & Anthropol Museum, Sofia, Bulgaria

[2] Bulgarian Acad Sci, Space & Solar Terr Res Inst, Sofia, Bulgaria

来源：

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2012年 / 26卷

关键词：

glycolipids; hypoxia; sodium nitrite; nuclear subcellular fraction; rat brain;

D O I：

10.5504/50YRTIMB.2011.0014

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, we report glycolipid changes in the nuclear subcellular fraction from hypoxic rat brains. Male Wistar rats at the age of three months were subjected to sodium nitrite-induced hypoxia. Te nuclear fraction was isolated and lipids were extracted. The glycolipid content was measured by spectrophotometry and thin-layer chromatography. In controls, gangliosides and cerebrosides were the major glycolipid classes. In the nuclear membrane of hypoxic brains, we found increased levels of total glycolipids (5.2-fold), gangliosides (4.1-fold), and cerebrosides (5.9-fold). These changes reflect the disturbances in the glycolipid turnover. Most probably they are associated with impaired energy metabolism. The accumulation of glycolipids may be interpreted as a physiological adaptive response to hypoxia.

引用

页码：77 / 79

页数：3

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