Enzymatic synthesis of β-N-(γ-L(+)-glutamyl)phenylhydrazine with Escherichia coli γ-glutamyltranspeptidase

被引：17

作者：

Zhang, Hongjuan ^{[1
,2
]}

Zhan, Yueping ^{[1
]}

Chang, Junjun ^{[1
]}

Liu, Junzhong ^{[1
]}

Xu, Lisheng ^{[1
]}

Wang, Zhiyuan ^{[1
]}

Liu, Qian ^{[1
]}

Jiao, Qingcai ^{[1
]}

机构：

[1] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Sch Pharm, Nanjing 210029, Jiangsu, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 10期

关键词：

beta-N-(gamma-L(+)-glutamyl)phenylhydrazine; gamma-Glutamyltranspeptidase; Phenylhydrazine; Reversible inhibition; AGARITINE; DERIVATIVES;

D O I：

10.1007/s10529-012-1000-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new method for the synthesis of beta-N-(gamma-l(+)-glutamyl)phenylhydrazine is presented. This compound was prepared from l-glutamine and phenylhydrazine through a transpeptidation reaction of Escherichia coli gamma-glutamyltranspeptidase although phenylhydrazine has been reported to be an inhibitor of the enzyme. The optimum reaction conditions were 60 mM l-glutamine, 300 mM phenylhydrazine, 40 U gamma-glutamyltranspeptidase/ml, and pH 9 in approx. 800 ml. After 6 h at 37 A degrees C, the product was obtained with a conversion rate of 93 % (mol/mol). gamma-Glutamyltranspeptidase was reversibly inhibited only when phenylhydrazine was above 300 mM.

引用

页码：1931 / 1935

页数：5

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