Distinct properties of cyclin-dependent kinase complexes containing cyclin A1 and cyclin A2

被引：17

作者：

Joshi, Ayesha R. ^{[1
,2
]}

Jobanputra, Vaidehi ^{[1
,2
]}

Lele, Karen M. ^{[1
,3
]}

Wolgemuth, Debra J. ^{[1
,2
,3
,4
]}

机构：

[1] Columbia Univ, Med Ctr, Dept Obstet & Gynaecol, New York, NY 10032 USA

[2] Columbia Univ, Med Ctr, Dept Gen & Dev, New York, NY 10032 USA

[3] Columbia Univ, Med Ctr, Inst Human Nutr, New York, NY 10032 USA

[4] Columbia Univ, Med Ctr, Herbert Irving Comprehens Canc Ctr, New York, NY 10032 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 378卷 / 03期

关键词：

Cyclin A1; Cyclin A2; CDK1; CDK2; CELL-CYCLE; RETINOBLASTOMA PROTEIN; MALE MEIOSIS; GERM-CELLS; PHOSPHORYLATION; CDK2; ACTIVATION; MICE; P53; EXPRESSION;

D O I：

10.1016/j.bbrc.2008.11.077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The distinct expression patterns of the two A-type cyclins during spermatogenesis and the absolute requirement for cyclin A1 in this biological process in vivo suggest that they may confer distinct biochemical properties to their CDK partners. We therefore compared human cyclin A1- and cyclin A2-containing CDK complexes in vitro by determining kinetic constants and by examining the complexes for their ability to phosphorylate pRb and p53. Differences in biochemical activity were observed in CDK2 but not CDK1 when complexed with cyclin A1 versus cyclin A2. Further, CDK1/cyclin A1 is a better kinase complex for phosphorylating potentially physiologically relevant Substrates pRb and p53 than CDK2/cyclin A2. The activity of CDKs can therefore be regulated depending Upon which A-type cyclin they bind and CDK1/cyclin A1 might be preferred in vivo. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：595 / 599

页数：5

共 32 条

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