Single-cell trapping and impedance measurement utilizing dielectrophoresis in a parallel-plate microfluidic device

被引：70

作者：

Chen, Nai-Chin ^{[1
,2
]}

Chen, Chun-Hong ^{[1
,2
]}

Chen, Ming-Kun ^{[1
,2
]}

Jang, Ling-Sheng ^{[1
,2
]}

Wang, Min-Haw ^{[3
]}

机构：

[1] Natl Cheng Kung Univ, Dept Elect Engn, Tainan 70101, Taiwan

[2] Natl Cheng Kung Univ, Ctr Micro Nano Sci & Technol, Tainan 70101, Taiwan

[3] Chinese Culture Univ, Dept Elect Engn, Taipei, Taiwan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 190卷

关键词：

Dielectrophoresis (DEP); Liquid dielectrophoresis (LDEP); Single cells; ACTUATION; DROPLETS;

D O I：

10.1016/j.snb.2013.08.104

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper integrates fluid manipulation, single-cell trapping, and single-cell impedance measurement into a parallel-plate chip. Liquid dielectophoresis (LDEP) and dielectrophoresis (DEP) are utilized to manipulate a sucrose solution and HeLa cells in a chip, respectively. The sucrose solution with HeLa cells is transported by LDEP. A cell is trapped by DEP and then its impedance is measured in an SU-8 cavity between measurement electrodes. Finite element analysis (FEA) is used to optimize the microstructure of the SU-8 cavity. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：570 / 577

页数：8

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