Single-cell trapping and impedance measurement utilizing dielectrophoresis in a parallel-plate microfluidic device

被引：68
作者：
Chen, Nai-Chin [1 ,2 ]
Chen, Chun-Hong [1 ,2 ]
Chen, Ming-Kun [1 ,2 ]
Jang, Ling-Sheng [1 ,2 ]
Wang, Min-Haw [3 ]
机构：
[1] Natl Cheng Kung Univ, Dept Elect Engn, Tainan 70101, Taiwan
[2] Natl Cheng Kung Univ, Ctr Micro Nano Sci & Technol, Tainan 70101, Taiwan
[3] Chinese Culture Univ, Dept Elect Engn, Taipei, Taiwan
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 190卷
关键词：
Dielectrophoresis (DEP); Liquid dielectrophoresis (LDEP); Single cells; ACTUATION; DROPLETS;
D O I：
10.1016/j.snb.2013.08.104
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This paper integrates fluid manipulation, single-cell trapping, and single-cell impedance measurement into a parallel-plate chip. Liquid dielectophoresis (LDEP) and dielectrophoresis (DEP) are utilized to manipulate a sucrose solution and HeLa cells in a chip, respectively. The sucrose solution with HeLa cells is transported by LDEP. A cell is trapped by DEP and then its impedance is measured in an SU-8 cavity between measurement electrodes. Finite element analysis (FEA) is used to optimize the microstructure of the SU-8 cavity. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：570 / 577
页数：8
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