Duplex real-time PCR for the determination of non-Basmati rice in Basmati rice (Oryza sativa)

被引：8
|
作者：
Bucher, Thomas B. [1 ]
Fridez, Francoise [2 ]
Koeppel, Rene [1 ]
机构：
[1] Food Control Author Canton Zurich, Zurich, Switzerland
[2] Food Control Author Canton Neuchatel, Neuchatel, Switzerland
来源：
EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2014年 / 238卷 / 03期
关键词：
Duplex; Microsatelite; PCR; Basmati; Rice; Plexor (TM) PCR; QUANTIFICATION; ADULTERATION;
D O I：
10.1007/s00217-013-2119-2
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Basmati rice has a higher trade value compared to other rice varieties. Adulterations of Basmati with cheaper varieties, sometimes in substantially amounts, are well known. To screen Basmati samples for such fraud, an efficient duplex real-time PCR assay was developed. It applies a mixed technique using hydrolysis probes in conjunction with Plexor (TM) technology. This assay is a cost-effective system to screen for non-Basmati contents in the range of 1 % or higher. It is cheaper and less time consuming than microsatellite analysis which is the conventional method applied, and comparing the two methods reveals good correlation.
引用
收藏
页码：417 / 423
页数：7
相关论文
共 50 条
  • [21] Aroma volatile analyses and 2AP characterization at various developmental stages in Basmati and Non-Basmati scented rice (Oryza sativa L.) cultivars
    Vidya R. Hinge
    Hemant B. Patil
    Altafhusain B. Nadaf
    Rice, 2016, 9
  • [22] Comparative Characterization of Aroma Volatiles and Related Gene Expression Analysis at Vegetative and Mature Stages in Basmati and Non-Basmati Rice (Oryza sativa L.) Cultivars
    Vidya Hinge
    Hemant Patil
    Altafhusain Nadaf
    Applied Biochemistry and Biotechnology, 2016, 178 : 619 - 639
  • [23] Comparative Characterization of Aroma Volatiles and Related Gene Expression Analysis at Vegetative and Mature Stages in Basmati and Non-Basmati Rice (Oryza sativa L.) Cultivars
    Hinge, Vidya
    Patil, Hemant
    Nadaf, Altafhusain
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2016, 178 (04) : 619 - 639
  • [24] Diversity Analysis in Selected Non-basmati Scented Rice Collection
    Mathure, Sarika
    Jawali, Narendra
    Nadaf, Altafhusain
    RICE SCIENCE, 2010, 17 (01) : 35 - 42
  • [25] Irrigation schedule in producing quality basmati rice (Oryza sativa)
    Bali, AS
    Uppal, HS
    INDIAN JOURNAL OF AGRICULTURAL SCIENCES, 1999, 69 (05): : 325 - 328
  • [26] Development of a simple functional marker for fragrance in rice and its validation in Indian Basmati and non-Basmati fragrant rice varieties
    Sakthivel, K.
    Rani, N. Shobha
    Pandey, Manish K.
    Sivaranjani, A. K. P.
    Neeraja, C. N.
    Balachandran, S. M.
    Madhav, M. Sheshu
    Viraktamath, B. C.
    Prasad, G. S. V.
    Sundaram, R. M.
    MOLECULAR BREEDING, 2009, 24 (02) : 185 - 190
  • [27] Diversity in phenolics, amino acids, rheology and noodles glycemic response of brown rice from non-basmati and basmati rice
    Mudgal, Swasti
    Singh, Narpinder
    FOOD RESEARCH INTERNATIONAL, 2022, 158
  • [28] Development of a simple functional marker for fragrance in rice and its validation in Indian Basmati and non-Basmati fragrant rice varieties
    K. Sakthivel
    N. Shobha Rani
    Manish K. Pandey
    A. K. P. Sivaranjani
    C. N. Neeraja
    S. M. Balachandran
    M. Sheshu Madhav
    B. C. Viraktamath
    G. S. V. Prasad
    R. M. Sundaram
    Molecular Breeding, 2009, 24 : 185 - 190
  • [29] PHYSICOCHEMICAL, TEXTURAL, PASTING, AND IN VITRO DIGESTION PROPERTIES OF SOME BASMATI AND NON-BASMATI RICE CULTIVARS
    Kaur, Shubhneet
    Panesar, Parmjit S.
    Bera, Manab B.
    Kumari, Shweta
    INTERNATIONAL JOURNAL OF FOOD PROPERTIES, 2014, 17 (05) : 1055 - 1066
  • [30] Sustainability and impact assessment of IPM in Basmati rice (Oryza sativa)
    Tanwar, R. K.
    Singh, S.
    Singh, S. P.
    Khokhar, M. K.
    Kanwar, V
    INDIAN JOURNAL OF AGRICULTURAL SCIENCES, 2022, 92 (04): : 450 - 454
12345