Duplex real-time PCR for the determination of non-Basmati rice in Basmati rice (Oryza sativa)

被引：8
|
作者：
Bucher, Thomas B. [1 ]
Fridez, Francoise [2 ]
Koeppel, Rene [1 ]
机构：
[1] Food Control Author Canton Zurich, Zurich, Switzerland
[2] Food Control Author Canton Neuchatel, Neuchatel, Switzerland
来源：
EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2014年 / 238卷 / 03期
关键词：
Duplex; Microsatelite; PCR; Basmati; Rice; Plexor (TM) PCR; QUANTIFICATION; ADULTERATION;
D O I：
10.1007/s00217-013-2119-2
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Basmati rice has a higher trade value compared to other rice varieties. Adulterations of Basmati with cheaper varieties, sometimes in substantially amounts, are well known. To screen Basmati samples for such fraud, an efficient duplex real-time PCR assay was developed. It applies a mixed technique using hydrolysis probes in conjunction with Plexor (TM) technology. This assay is a cost-effective system to screen for non-Basmati contents in the range of 1 % or higher. It is cheaper and less time consuming than microsatellite analysis which is the conventional method applied, and comparing the two methods reveals good correlation.
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页码：417 / 423
页数：7
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