Development of polymorphic microsatellite markers for the North American porcupine, Erethizon dorsatum, using paired-end Illumina sequencing

被引：2

作者：

Barthelmess, Erika L. ^{[1
]}

Love, Cara N. ^{[2
]}

Jones, Kenneth L. ^{[3
]}

Lance, Stacey L. ^{[2
]}

机构：

[1] St Lawrence Univ, Dept Biol, Canton, NY 13617 USA

[2] Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA

[3] Univ Colorado Sch Med, Dept Biochem & Mol Genet, Aurora, CO 80045 USA

来源：

CONSERVATION GENETICS RESOURCES | 2013年 / 5卷 / 04期

基金：

美国安德鲁·梅隆基金会;

关键词：

Erethizon dorsatum; Porcupine; Illumina; Microsatellite; PAL_FINDER; PCR primers; SSR;

D O I：

10.1007/s12686-013-9933-5

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We isolated and characterized a total of 19 microsatellite loci from the North American porcupine, Erethizon dorsatum. Loci were screened in 22 individuals from St. Lawrence and Franklin Counties in northern New York State. The number of alleles per locus ranged from 4 to 14, observed heterozygosity ranged from 0.316 to 1.000, and the probability of identity values ranged from 0.028 to 0.243. These new loci will provide tools for examination of the effects of habitat fragmentation, for instance by roads and other infrastructure, on gene flow and population subdivision in this species.

引用

页码：925 / 927

页数：3

共 7 条

[1] The influence of body-size and diet on road-kill trends in mammals
Barthelmess, Erika L.
Brooks, Margot S.
[J]. BIODIVERSITY AND CONSERVATION, 2010, 19 (06) : 1611 - 1629
[2] Rapid Microsatellite Identification from Illumina Paired-End Genomic Sequencing in Two Birds and a Snake
Castoe, Todd A.
Poole, Alexander W.
de Koning, A. P. Jason
Jones, Kenneth L.
Tomback, Diana F.
Oyler-McCance, Sara J.
Fike, Jennifer A.
Lance, Stacey L.
Streicher, Jeffrey W.
Smith, Eric N.
Pollock, David D.
[J]. PLOS ONE, 2012, 7 (02):
[3] TOUCHDOWN PCR TO CIRCUMVENT SPURIOUS PRIMING DURING GENE AMPLIFICATION
DON, RH
COX, PT
WAINWRIGHT, BJ
BAKER, K
MATTICK, JS
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (14) : 4008 - 4008
[4] Development and characterization of sixteen microsatellite markers for the federally endangered species: Leptodea leptodon (Bivalvia: Unionidae) using paired-end Illumina shotgun sequencing
O'Bryhim, Jason
Chong, Jer Pin
Lance, Stacey L.
Jones, Kenneth L.
Roe, Kevin J.
[J]. CONSERVATION GENETICS RESOURCES, 2012, 4 (03) : 787 - 789
[5] GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research
Peakall, R
Smouse, PE
[J]. MOLECULAR ECOLOGY NOTES, 2006, 6 (01): : 288 - 295
[6] GENEPOP′007:: a complete re-implementation of the GENEPOP software for Windows and Linux
Rousset, Francois
[J]. MOLECULAR ECOLOGY RESOURCES, 2008, 8 (01): : 103 - 106
[7] Wilson, 2005, MAMMAL SPECIES WORLD

← 1 →