The conjugation of rhodamine B enables carrier-free mitochondrial delivery of functional proteins

被引:10
|
作者
Shi, Jiayuan [1 ]
Zhao, Dan [1 ]
Li, Xiang [1 ]
Ding, Feng [1 ]
Tang, Xuemei [1 ]
Liu, Nian [2 ]
Huang, Hua [1 ]
Liu, Changlin [1 ]
机构
[1] Cent China Normal Univ, Sch Chem, Int Joint Res Ctr Intelligent Biosensing Technol, Minist Educ,Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China
[2] Wuhan Univ, Sch Basic Med Sci, Dept Biomed Engn, Wuhan 430071, Peoples R China
基金
中国国家自然科学基金;
关键词
INTRACELLULAR DELIVERY; SUPEROXIDE-DISMUTASE; DRUG TRANSPORTERS; HYDROGEN-PEROXIDE; CANCER-CELLS; PROLIFERATION; EXPRESSION; DESIGN; OCTN2; REDOX;
D O I
10.1039/d0ob01305f
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
The development of protein-based therapeutics faces many challenges, for example, carrier-dependence, safety concerns, endocytosis-dependence, and uncertain in vivo therapeutic outcomes. Small molecules are rarely used for intracellular organelle-targeting and disease tissue-specific carrier-independent delivery of therapeutic proteins. Here, we report that rhodamine B, after modification with proteins, is able to guide carrier-free delivery into mitochondria and tissue-dependent distributions of functional proteins through organic cation transporters (OCTs). The enrichment of the modified catalase in the cancer tissue efficiently suppresses xenograft human lung tumor in mice. This carrier-free delivery platform of proteins may emerge as a simple yet powerful approach for cancer treatment.
引用
收藏
页码:6829 / 6839
页数:11
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