Preparation of Laccase Immobilized Cryogels and Usage for Decolorization

被引：25

作者：

Uygun, Murat ^{[1
]}

机构：

[1] Adnan Menderes Univ, Kocarli Vocat & Training Sch, TR-09970 Aydin, Turkey

来源：

JOURNAL OF CHEMISTRY | 2013年 / 2013卷

关键词：

REACTIVE DYES; SYNTHETIC DYES; TEXTILE DYES; PURIFICATION; INVERTASE; MEDIATORS;

D O I：

10.1155/2013/387181

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Poly(methyl methacrylate-co-glycidyl methacrylate) (poly(MMA-co-GMA)) cryogels were synthesized by radical cryopolymerization technique. Then, laccase enzyme was covalently attached to the cryogel and characterized by using swelling studies and SEM and EDX analyses. Kinetic properties and optimum conditions of the immobilized and free laccase were studied and it was found that V-max of the immobilized laccase was lower than that of free laccase K-m of the immobilized laccase was increased upon immobilization. Optimum pH was found to be 4.0 for each type of laccase, while optimum temperature was shifted to the warmer region after the immobilization. It was also found that thermal stability of the immobilized laccase was higher than that of free laccase. Immobilized laccase could be used for 10 times successive reuse with no significant decrease in its activity. Also, these laccase immobilized cryogels were successfully used for the decolorization of seven different dyes.

引用

页数：7

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