Imaging flow cytometry enhances particle detection sensitivity for extracellular vesicle analysis

被引：19
|
作者：
Clark, Robin T. [1 ]
机构：
[1] EMD Millipore Corp, Billerica, MA 01821 USA
来源：
NATURE METHODS | 2015年 / 12卷 / 04期
关键词：
D O I：
10.1038/nmeth.f.380
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The study of extracellular vesicles (EVs) has evolved into a compelling field of intercellular communication and pathophysiology research. The submicron size of EVs and the complexity of the biofluids in which they are suspended confound their analysis by traditional light microscopy or flow cytometry. Observation and quantitation of EVs have been achieved with Amnis (R) imaging flow cytometers (IFCs) with reduced volumes and sample preparation times, suggesting an auspicious role for IFCs in the advancement of EV biology.
引用
收藏
页码：i / ii
页数：2
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