A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-Chitosan

被引：3

作者：

Diao, Qizhi ^{[2
]}

Li, Yuan ^{[2
]}

Zhou, Mi ^{[1
]}

Xie, Guoming ^{[1
]}

机构：

[1] Chongqing Med Univ, Key Lab Lab Med Diagnost, Minist Educ, Dept Lab Med, Chongqing, Peoples R China

[2] Chongqing Med Univ YongChuan, Clin Lab, Yongchuan Hosp, Chongqing, Peoples R China

来源：

MATERIALS FOR ENVIRONMENTAL PROTECTION AND ENERGY APPLICATION, PTS 1 AND 2 | 2012年 / 343-344卷

关键词：

Immunosensor; myeloperoxidase; self-assemble; multi- walled carbon nanotubes; horseradish peroxidase;

D O I：

10.4028/www.scientific.net/AMR.343-344.1207

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

A new electrochemical immunosensor for scrum myeloperoxidase (MPO) has been developed based on the self-assembly multilays of multi-walled carbon nanotubes (MWNTs), thionine (THI), gold nanoparticles (GNPs) and chitosanon (CHIT) on the glassy carbon electrode (GCE). The antibody of MPO (anti-MPO) was absorbed on the surface of GNPs monolayer. Horseradish peroxidase (HRP) was employed to block non-specific binding and amplify the response current signal. It was observed that the peak current was linear with the MPO concentration in a range of 2.5-125 mu gl-l. The detection limit was 1.425 mu gl-l (S/N=3). Correlation analysis showed that this new immunosensor assay has a significant correlation with enzyme-linked immunosorbent assay (ELISA) (r=-0.96, p>0.05) for 40 clinical specimens.

引用

页码：1207 / +

页数：2

共 11 条

[1] Alternate assemblies of thionine and Au-nanoparticles on an amino functionalized surface [J].

Cheng, WL ;

Jiang, JG ;

Dong, SJ ;

Wang, E .

CHEMICAL COMMUNICATIONS, 2002, (16) :1706-1707

[2] CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS [J].

FRENS, G .

NATURE-PHYSICAL SCIENCE, 1973, 241 (105) :20-22

[3] Synthesis of chitosan-stabilized gold nanoparticles in the absence/presence of tripolyphosphate [J].

Huang, HZ ;

Yang, XR .

BIOMACROMOLECULES, 2004, 5 (06) :2340-2346

[4]

Iijima S., 1991, NATURE, V241, P20

[5] A simple and versatile self-assembled monolayer based surface plasmon resonance immunosensor for highly sensitive detection of 2,4-D from natural water resources [J].

Kim, Sook Jin ;

Gobi, K. Vengatajalabathy ;

Tanaka, Hiroyuki ;

Shoyama, Yukihiro ;

Miura, Norio .

SENSORS AND ACTUATORS B-CHEMICAL, 2008, 130 (01) :281-289

[6]

Li Shi-hong, 2007, Zhonghua Xinxueguanbing Zazhi, V35, P241

[7] Myeloperoxidase: A new biomarker of inflammation in ischemic heart disease and acute coronary syndromes [J].

Loria, Valentina ;

Dato, Ilaria ;

Graziani, Francesca ;

Biasucci, Luigi M. .

MEDIATORS OF INFLAMMATION, 2008, 2008

[8] Layer-by-layer assembled multilayer films of titanate nanotubes, Ag- or Au-loaded nanotubes, and nanotubes/nanosheets with polycations [J].

Ma, RZ ;

Sasaki, T ;

Bando, Y .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (33) :10382-10388

[9] Serum Myeloperoxidase levels are associated with the future risk of coronary artery disease in apparently healthy individuals - The EPIC-Norfolk prospective population study [J].

Meuwese, Marijn C. ;

Stroes, Erik S. G. ;

Hazen, Stanley L. ;

van Miert, Joram N. ;

Kuivenhoven, Jan Albert ;

Schaub, Robert G. ;

Wareham, Nicholas J. ;

Luben, Robert ;

Kastelein, John J. P. ;

Khaw, Kay-Tee ;

Boekholdt, S. Matthijs .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2007, 50 (02) :159-165

[10] Concurrent evaluation of novel cardiac biomarkers in acute coronary syndrome: myeloperoxidase and soluble CD40 ligand and the risk of recurrent ischaemic events in TACTICS-TIMI 18 [J].

Morrow, David A. ;

Sabatine, Marc S. ;

Brennan, Marie-Luise ;

de Lemos, James A. ;

Murphy, Sabina A. ;

Ruff, Christian T. ;

Rifai, Nader ;

Cannon, Christopher P. ;

Hazen, Stanley L. .

EUROPEAN HEART JOURNAL, 2008, 29 (09) :1096-1102

← 1 2 →