A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-Chitosan

被引:3
作者
Diao, Qizhi [2 ]
Li, Yuan [2 ]
Zhou, Mi [1 ]
Xie, Guoming [1 ]
机构
[1] Chongqing Med Univ, Key Lab Lab Med Diagnost, Minist Educ, Dept Lab Med, Chongqing, Peoples R China
[2] Chongqing Med Univ YongChuan, Clin Lab, Yongchuan Hosp, Chongqing, Peoples R China
来源
MATERIALS FOR ENVIRONMENTAL PROTECTION AND ENERGY APPLICATION, PTS 1 AND 2 | 2012年 / 343-344卷
关键词
Immunosensor; myeloperoxidase; self-assemble; multi- walled carbon nanotubes; horseradish peroxidase;
D O I
10.4028/www.scientific.net/AMR.343-344.1207
中图分类号
TE [石油、天然气工业]; TK [能源与动力工程];
学科分类号
0807 ; 0820 ;
摘要
A new electrochemical immunosensor for scrum myeloperoxidase (MPO) has been developed based on the self-assembly multilays of multi-walled carbon nanotubes (MWNTs), thionine (THI), gold nanoparticles (GNPs) and chitosanon (CHIT) on the glassy carbon electrode (GCE). The antibody of MPO (anti-MPO) was absorbed on the surface of GNPs monolayer. Horseradish peroxidase (HRP) was employed to block non-specific binding and amplify the response current signal. It was observed that the peak current was linear with the MPO concentration in a range of 2.5-125 mu gl-l. The detection limit was 1.425 mu gl-l (S/N=3). Correlation analysis showed that this new immunosensor assay has a significant correlation with enzyme-linked immunosorbent assay (ELISA) (r=-0.96, p>0.05) for 40 clinical specimens.
引用
收藏
页码:1207 / +
页数:2
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