Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18

被引：31

作者：

Suo, Siqingaowa ^{[1
,2
]}

Ren, Yudong ^{[1
]}

Li, Guangxing ^{[1
]}

Zarlenga, Dante ^{[3
]}

Bu, Ri-e ^{[2
]}

Su, Dingding ^{[4
]}

Li, Xunliang ^{[1
]}

Li, Pengchong ^{[1
]}

Meng, Fandan ^{[1
]}

Wang, Chao ^{[1
]}

Ren, Xiaofeng ^{[1
,2
]}

机构：

[1] NE Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China

[2] Inner Mongolia Univ Nationalities, Sch Life Sci, Tongliao 028043, Inner Mongolia, Peoples R China

[3] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA

[4] Hunan Agr Univ, Hunan Prov Key Lab Germplasm Innovat & Utilizat G, Changsha 410128, Hunan, Peoples R China

来源：

VIRUS RESEARCH | 2012年 / 167卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Porcine epidemic diarrhea virus; Spike protein; Porcine interleukin-18 gene; Adjuvant; DNA immunization; TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS; MESSENGER-RNA EXPRESSION; T-CELLS; OSTERTAGIA-OSTERTAGI; INTERFERON-GAMMA; IFN-GAMMA; CYTOKINE; VIRUS; INFECTION; INTERLEUKIN-18;

D O I：

10.1016/j.virusres.2012.05.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea, a highly contagious enteric disease of swine. The Spike (S) protein is one of the main structural proteins of PEDV capable of inducing neutralizing antibodies in vivo. Herein, we generated three distinct DNA constructs in the eukaryotic expression plasmid pVAX1; one encoding the S protein [pVAX1-(PEDV-S)], the second encoding the N-terminal fragment (S1) [pVAX1-(PEDV-S1)] containing potent antigenic sites, and the third expressing the porcine interleukin-18 (pIL-18) [pVAX1-(IL-18)]. Immunofluorescence assays in BHK-21 cells demonstrated successful protein expression from all 3 constructs. Kunming mice were injected separately with each of these constructs or with a pVAX1-(PEDV-S1)/pVAX1-(IL-18) combination, an attenuated PEDV vaccine, or vector only control. Animals were examined for T lymphocyte proliferation, anti-PEDV antibodies, IFN-gamma and IL-4 protein levels, and cytotoxic T cell function in mouse peripheral blood and spleen. In all cases, results showed that pVAX1-(PEDV-S) and the combination of pVAX1-(PEDV-S1) with pVAX1-( IL-18) induced the strongest responses; however, pIL-18 had no adjuvant effects when given in combination with pVAX1-(PEDV-S1). (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：259 / 266

页数：8

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