Perioperative Search for Circulating Tumor Cells in Patients Undergoing Prostate Brachytherapy for Clinically Nonmetastatic Prostate Cancer

被引:24
作者
Tsumura, Hideyasu [1 ]
Satoh, Takefumi [1 ]
Ishiyama, Hiromichi [2 ]
Tabata, Ken-ichi [1 ]
Takenaka, Kouji [2 ]
Sekiguchi, Akane [2 ]
Nakamura, Masaki [3 ]
Kitano, Masashi [2 ]
Hayakawa, Kazushige [2 ]
Iwamura, Masatsugu [1 ]
机构
[1] Kitasato Univ, Sch Med, Dept Urol, Sagamihara, Kanagawa 2520374, Japan
[2] Kitasato Univ, Dept Radiol & Radiat Oncol, Sch Med, Sagamihara, Kanagawa 2520374, Japan
[3] Kitasato Univ, Sch Allied Hlth Sci, Dept Microbiol, Sagamihara, Kanagawa 2520373, Japan
关键词
prostate cancer; brachytherapy; circulating tumor cell; EXTERNAL-BEAM RADIOTHERAPY; DOSE-RATE BRACHYTHERAPY; TOUCH ISOLATION TECHNIQUE; CUSTOM-LINKED SEEDS; HIGH-RISK; RADICAL PROSTATECTOMY; PERIPHERAL-BLOOD; FOLLOW-UP; OUTCOMES; DISSEMINATION;
D O I
10.3390/ijms18010128
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Despite the absence of local prostate cancer recurrence, some patients develop distant metastases after prostate brachytherapy. We evaluate whether prostate brachytherapy procedures have a potential risk for hematogenous spillage of prostate cancer cells. Fifty-nine patients who were undergoing high-dose-rate (HDR) or low-dose-rate (LDR) brachytherapy participated in this prospective study. Thirty patients with high-risk or locally advanced cancer were treated with HDR brachytherapy after neoadjuvant androgen deprivation therapy (ADT). Twenty-nine patients with clinically localized cancer were treated with LDR brachytherapy without neoadjuvant ADT. Samples of peripheral blood were drawn in the operating room before insertion of needles (preoperative) and again immediately after the surgical manipulation (intraoperative). Blood samples of 7.5 mL were analyzed for circulating tumor cells (CTCs) using the CellSearch System. While no preoperative samples showed CTCs (0%), they were detected in intraoperative samples in 7 of the 59 patients (11.8%; preoperative vs. intraoperative, p = 0.012). Positive CTC status did not correlate with perioperative variables, including prostate-specific antigen (PSA) at diagnosis, use of neoadjuvant ADT, type of brachytherapy, Gleason score, and biopsy positive core rate. We detected CTCs from samples immediately after the surgical manipulation. Further study is needed to evaluate whether those CTCs actually can survive and proliferate at distant sites.
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页数:10
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