Comparison of Autophagy mRNA Expression between Chronic Otitis Media With and Without Cholesteatoma

被引:4
作者
Jung, Junyang [1 ]
Jung, Su Young [3 ]
Kim, Myung Gu [4 ]
Kim, Young Il [5 ]
Kim, Sang Hoon [2 ]
Yeo, Seung Geun [2 ,5 ]
机构
[1] Kyung Hee Univ, Sch Med, Dept Anat & Neurobiol, Seoul, South Korea
[2] Kyung Hee Univ, Sch Med, Dept Otorhinolaryngol, Seoul, South Korea
[3] Hanyang Univ, Myongji Hosp, Dept Otorhinolaryngol Head & Neck Surg, Coll Med, Goyang, South Korea
[4] Sungkyunkwan Univ, Samsung Changwon Hosp, Dept Otorhinolaryngol, Sch Med, Chang Won, South Korea
[5] Kyung Hee Univ, Med Sci Res Inst, Med Ctr, Seoul, South Korea
基金
新加坡国家研究基金会;
关键词
Autophagy; Otitis media; Cholesteatoma; BONE-RESORPTION; HEALTH; INTERLEUKIN-1-ALPHA; ETIOPATHOGENESIS; BACTERIOLOGY; PATHOGENESIS; DISEASE;
D O I
10.7874/jao.2020.00108
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
Background and Objectives: Autophagy is known to be associated with pathogen infection. However, the expression of autophagy-related proteins has not been studied in chronic otitis media without cholesteatoma (COM) or with cholesteatoma (CholeOM). This study aimed to determine whether there is a difference between COM and CholeOM in autophagy-related gene mRNA expression. Subjects and Methods: For 47 patients with chronic otitis media, the inflammatory tissues were classified into granulation tissue (COM) or cholesteatoma (CholeOM) according to biopsy results. Results: PI3K mRNA expression (COM vs. CholeOM, mean +/- SD, 0.009 +/- 0.010 vs. 0.003 +/- 0.004; p=0.004) was lower, whereas Beclin-1 mRNA expression (0.089 +/- 0.107 vs. 0.176 +/- 0.163; p=0.034) was higher in the CholeOM group. Expression of PI3K mRNA in the CholeOM group was lower than that in the COM subgroups with presence of bacteria (0.022 +/- 0.019 vs. 0.001 +/- 0.001; p=0.001), otorrhea (0.049 +/- 0.068 vs. 0.003 +/- 0.004; p=0.004), and hearing loss over 40 dB (0.083 +/- 0.130 vs. 0.003 +/- 0.004; p=0.005). Conclusions: The data suggested that different autophagy proteins play important roles in chronic otitis media according to the presence or absence of cholesteatoma.
引用
收藏
页码:191 / 197
页数:7
相关论文
共 30 条
  • [1] FURTHER STUDIES ON A COLLAGENASE IN MIDDLE EAR CHOLESTEATOMA
    ABRAMSON, M
    GROSS, J
    [J]. ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 1971, 80 (02) : 177 - +
  • [2] INTERLEUKIN-1 CAUSING BONE DESTRUCTION IN MIDDLE-EAR CHOLESTEATOMA
    AHN, JM
    HUANG, CC
    ABRAMSON, M
    [J]. OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 1990, 103 (04) : 527 - 536
  • [3] Mammalian Autophagy: How Does It Work?
    Bento, Carla F.
    Renna, Maurizio
    Ghislat, Ghita
    Puri, Claudia
    Ashkenazi, Avraham
    Vicinanza, Mariella
    Menzies, Fiona M.
    Rubinsztein, David C.
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, VOL 85, 2016, 85 : 685 - 713
  • [4] Understanding the aetiology and resolution of chronic otitis media from animal and human studies
    Bhutta, Mahmood F.
    Thornton, Ruth B.
    Kirkham, Lea-Ann S.
    Kerschner, Joseph E.
    Cheeseman, Michael T.
    [J]. DISEASE MODELS & MECHANISMS, 2017, 10 (11) : 1289 - 1300
  • [5] ISOLATED OSTEOCLASTS RESORB THE ORGANIC AND INORGANIC COMPONENTS OF BONE
    BLAIR, HC
    KAHN, AJ
    CROUCH, EC
    JEFFREY, JJ
    TEITELBAUM, SL
    [J]. JOURNAL OF CELL BIOLOGY, 1986, 102 (04) : 1164 - 1172
  • [6] BROOK I, 1981, LARYNGOSCOPE, V91, P250
  • [7] Autophagy in Human Health and Disease
    Doria, Andrea
    Gatto, Mariele
    Punzi, Leonardo
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2013, 368 (19) : 1845 - 1845
  • [9] Chung JW, 1998, ACTA OTO-LARYNGOL, V118, P386
  • [10] Modulation of phagosome biogenesis by Legionella pneumophila creates an organelle permissive for intracellular growth
    Coers, J
    Monahan, C
    Roy, CR
    [J]. NATURE CELL BIOLOGY, 1999, 1 (07) : 451 - 453