Micromechanical PDGF recognition via lab-on-a-disc aptasensor arrays

被引：11

作者：

Bosco, F. G. ^{[1
]}

Bache, M. ^{[1
]}

Yang, J. ^{[2
]}

Chen, C. H. ^{[3
]}

Hwu, E. -T. ^{[3
]}

Lin, Q. ^{[2
]}

Boisen, A. ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Micro & Nanotechnol, DK-2800 Lyngby, Denmark

[2] Columbia Univ, Dept Mech Engn, New York, NY 10027 USA

[3] Acad Sinica, Inst Phys, Taipei 11529, Taiwan

来源：

SENSORS AND ACTUATORS A-PHYSICAL | 2013年 / 195卷

关键词：

Platelet derived growth factor; Biosensing; Cantilevers; DVD-ROM; Lab-on-a-disc; Protein detection; GROWTH-FACTOR; DIAGNOSTICS; MOLECULES; APTAMERS; BIOASSAY; SENSORS; LIGANDS; PROTEIN; PICKUP;

D O I：

10.1016/j.sna.2012.06.030

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A plug-and-play CD-like platform is used to perform a statistical detection of platelet derived growth factor (PDGF) proteins through aptamer-based surface functionalization of multiple microcantilever arrays. When PDGF proteins bind to aptamer coatings, the cantilevers deflect. The deflection response is monitored by optical read-out units from a commercial DVD-ROM device. We report on the use of an improved sensing platform which facilitates measurements under continuous liquid flow and with temperature control. Also, the mechanical wobbling of the DVD-ROM platform has been minimized and the scanning system has been optimized in order to detect cantilever deflections in liquid with nanometer scale resolution. The capability of the sensing platform is demonstrated by detection of clinically relevant concentrations of PDGF proteins. We present statistical measurements on 100 microcantilevers at different concentrations of PDGF, ranging from 10 nM to 400 nM. Hereby it is possible to reliably characterize the averaged mechanical response of cantilevers as a function of protein concentration. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：154 / 159

页数：6

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