Chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxB) in rhizobacteria isolated from crop plants

被引：50

作者：

Anandham, Rangasamy ^{[1
,2
]}

Indiragandhi, Pandiyan ^{[1
]}

Madhaiyan, Munusamy ^{[1
]}

Ryu, Kyoung Yul ^{[2
]}

Jee, Hyeong Jin ^{[2
]}

Sa, Tong Min ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Dept Agr Chem, Cheongju 361763, Chungbuk, South Korea

[2] Natl Inst Agr Sci & Technol, Organ Farming Div, Suwon 441707, South Korea

来源：

RESEARCH IN MICROBIOLOGY | 2008年 / 159卷 / 9-10期

关键词：

Paracoccus sulfur oxidation pathway; Rhizosphere; soxB; Thiosulfate-oxidizing bacteria; S4 intermediate pathway;

D O I：

10.1016/j.resmic.2008.08.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Twenty-one thiosulfate-oxidizing bacteria were isolated from rhizosphere soils and 16S rRNA analysis revealed that the isolates were affiliated with seven different phylogenetic groups within the Beta and Gamma subclasses of Proteobacteria and Actinobacteria. Among these, five genera, including Dyella, Burkholderia, Alcaligenes, Microbacterium and Leifsonia sp., represented new sulfur oxidizers in rhizosphere soils. The thiosulfate-oxidizing Dyella, Burkholderia, Alcaligenes, Microbacterium, Leifsonia and Pandoraea were able to grow chemolithotrophically with a medium containing thiosulfate and exhibited growth coupled with thiosulfate oxidation. They accumulated intermediate products such as sulfur, sulfite and trithionate in the spent medium during the time course of thiosulfate oxidation, and these products were finally oxidized into sulfate. Furthermore, they possessed thiosulfate-metabolizing enzymes such as rhodanese, thiosulfate oxidase, sulfite oxidase and trithionate hydrolase, suggesting that these bacteria use the 'S4 intermediate' (S4I) pathway for thiosulfate oxidation. Phylogenetic analysis of the soxB gene revealed that Pandoraea sp. and Pandoraea pnomenusa strains formed a separate lineage within Betaproteobacteria. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：579 / 589

页数：11

共 36 条

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