Molecular and morphological characterization of Pristionchus pacificus (Nematoda: Rhabditida: Neodiplogastridae), a new record of an entomophilic nematode from Iran

被引：5

作者：

Hassani-Kakhki, Mahnaz ^{[1
]}

Karimi, Javad ^{[1
]}

Shokoohi, Ebrahim ^{[2
]}

机构：

[1] Ferdowsi Univ Mashhad, Coll Agr, Dept Plant Protect, Biocontrol & Insect Pathol Lab, Mashhad, Iran

[2] Shahid Bahonar Univ Kerman, Coll Agr, Dept Plant Protect, Kerman, Iran

来源：

BIOLOGIA | 2013年 / 68卷 / 05期

关键词：

Pristionchus pacificus; SEM; ITS; 18S rDNA; Iran; PHYLOGENY; EVOLUTION; STEINERNEMATIDAE; SUBSTITUTIONS; COMMUNITIES; DIVERSITY; REVEALS; ISLAND; DNA;

D O I：

10.2478/s11756-013-0232-0

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

During a survey of entomopathogenic nematodes from the Mashhad region, in northeastern Iran, an entomophilic nematode was isolated using the Galleria trap method. Morphological characterization showed that this isolate belongs to the family Neodiplogastridae. Detailed morphological and morphometric characterization including SEM data fit with Pristionchus pacificus Sommer, Carta, Kim et Sternberg, 1996, representing the first record of this genus and species from Iran. Iranian specimens of P. pacificus FUM 5 are characterized by the body length (0.69-0.83 mm), the stoma (6-10 mu m) bearing a dorsal tooth and a conical elongated tail (146-220 mu m). Molecular analyses using 18S and ITS rDNA genes further support this identification. Measurements, illustrations, and SEM photographs of the Iranian isolate are provided and the phylogenetic position of the species is discussed.

引用

页码：910 / 917

页数：8

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