Genetic characterisation of Toxoplasma gondii isolates from European beavers (Castor fiber) and European wildcats (Felis silvestris silvestris)

被引：23

作者：

Herrmann, D. C. ^{[1
]}

Wibbelt, G. ^{[2
]}

Goetz, M. ^{[3
]}

Conraths, F. J. ^{[1
]}

Schares, G. ^{[1
]}

机构：

[1] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Epidemiol, D-16868 Wusterhausen, Germany

[2] Leibniz Inst Zoo & Wildlife Res IZW, Res Grp Wildlife Dis, D-10315 Berlin, Germany

[3] Tech Univ Dresden, Inst Forest Bot & Forest Zool, D-01737 Tharandt, Germany

来源：

VETERINARY PARASITOLOGY | 2013年 / 191卷 / 1-2期

关键词：

Toxoplasma gondii; Beaver; Wild cat; Germany; PCR-RFLP typing; NEOSPORA-CANINUM; ANTIBODY PREVALENCE; COASTAL CALIFORNIA; DNA FRAGMENT; SEROPREVALENCE; IDENTIFICATION; INFECTION; CARNIVORES; VIRULENT; RODENTS;

D O I：

10.1016/j.vetpar.2012.08.026

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Six free-ranging European beavers (Castor fiber) from Berlin greater metropolitan area and twelve European wildcats (Felis silvestris silvestris) originating from the German Federal State of Saxony-Anhalt were found dead and their carcasses were submitted for necropsy. Brain and lung samples were analysed for the presence of Toxoplasma gondii DNA. Histopathologic analysis of one beaver revealed several cyst-like protozoal structures in parts of the brain. Tissue DNA isolated from all animal samples was analysed by a specific T. gondii-PCR. Two beavers and four wildcats tested T. gondii-positive. DNA of the parasites was further analysed by PCR-RFLP typing using nine markers (nSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, 1358, PK1 and Apico). Only T. gondii type II alleles were detected, except for the Apico locus, where type I alleles were observed in two isolates from beavers and in three from wild cats. The results of this study indicate that type II T. gondii (including type II variant strain) is the most common genotype infecting wildcats and beavers from Germany. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：108 / 111

页数：4

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