Molecular detection of adhesins genes and biofilm formation in methicillin resistant Staphylococcus aureus

被引：10

作者：

Bekir, Karima ^{[1
]}

Haddad, Omayma ^{[2
]}

Grissa, Mohammed ^{[1
]}

Chaieb, Kamel ^{[1
]}

Bakhrouf, Amina ^{[1
]}

Elgarssdi, Salem Ibrahim ^{[2
]}

机构：

[1] Fac Pharm, Lab Anal Treatment & Valuat Environm Pollutants &, St Avicenna 5000, Monastir, Tunisia

[2] Univ El Fateh, Dept Microbiol & Immunol, Fac Pharm, Tripoli, Libya

来源：

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2012年 / 6卷 / 23期

关键词：

Staphylococcus aureus; nasal carriage; biofilm; hydrophobicity; adhesin genes; COAGULASE-NEGATIVE STAPHYLOCOCCI; POLYSACCHARIDE INTERCELLULAR ADHESIN; FIBRONECTIN-BINDING PROTEINS; SLIME PRODUCTION; CHRONIC SINUSITIS; CLUMPING FACTOR; ICAD GENES; IN-VITRO; INFECTIONS; ADHERENCE;

D O I：

10.5897/AJMR11.1191

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Staphylococcus aureus has been identified as a major cause of nosocomial infections in hospitals. In this study, 41 S. aureus have been isolated from the nasal cavity of children in Libya. Congo red agar assay (CRA), semi quantitative adherence assay and microbial adhesion to solvent (MATS) test were used to study qualitative and quantitative biofilm formation of the isolated strains. Molecular detection of adhesion (icaA, icaD, fnbA, cna and clfA) genes was detected by polymerase chain reaction. Our results showed that 21 strains (51.2%) were slime producers on CRA plates. In a parallel way, 19 strains were slime producer on polystyrene plates. The MATS assay showed that all the studied strains have hydrophilic character. Molecular detection of adhesion genes revealed that 56.1% strains were icaA and icaD positives, 34.1% were cna positives, and 56.1% were fnbA positives. Moreover clfA gene was not detected in all the tested strains. This study confirms the adhesion ability and the presence of adhesion genes in the majority of the strains isolated from the nasal cavity of Libyan children.

引用

页码：4908 / 4917

页数：10

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