Combined extract of Moringa oleifera and Centella asiatica modulates oxidative stress and senescence in hydrogen peroxide-induced human dermal fibroblasts

被引：15

作者：

Abdul Hisam, Elly Ezlinda ^{[1
,2
]}

Rofiee, Mohd Salleh ^{[1
]}

Khalid, Aina Mardhia ^{[1
]}

Jalaluddin, Ahmad Firdaus ^{[1
]}

Mohamad Yusof, Mohd Izwan ^{[1
,2
]}

Idris, Muhd Hanis ^{[1
,2
]}

Ramli, Salfarina ^{[1
,2
]}

James, Richard Johari ^{[1
,2
]}

Jack Yoeng, Wee ^{[1
]}

Lay Kek, Teh ^{[1
,2
]}

Salleh, Mohd Zaki ^{[1
,2
]}

机构：

[1] Univ Teknol MARA Selangor, Integrat Pharmacogen Inst iPROMISE, Puncak Alam, Malaysia

[2] Univ Teknol MARA Selangor, Fac Pharm, Puncak Alam, Malaysia

来源：

TURKISH JOURNAL OF BIOLOGY | 2018年 / 42卷 / 01期

关键词：

Moringa oleifera; Centella asiatica; senescence; oxidative stress; antioxidant; ANTIOXIDANT ACTIVITY; DNA-DAMAGE; IN-VITRO; CELLULAR SENESCENCE; LEAVES; MECHANISMS; FLAVONOIDS; INDUCTION; APOPTOSIS; PHENOLICS;

D O I：

10.3906/biy-1708-23

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Moringa oleifera Lam. and Centella asiatica (L.) Urb. leaves have been previously reported to exhibit antioxidant activity. The objective of the present study is to determine the in vitro antioxidant activity of the combined extracts of M. oleifera and C. asiatica (TGT-PRIMAAGE) and its effect on hydrogen peroxide (H2O2)-induced oxidative stress in human dermal fibroblasts. TGTPRIMAAGE acted on the mechanism of hydrogen transfer as it showed scavenging activity in the DPPH assay. This is due to the presence of phenolics and flavonoids in TGT-PRIMAAGE. TGT-PRIMAAGE effectively reduced cellular generation of reactive oxygen species induced by H2O2. The activities of superoxide dismutase and catalase were also increased in cells treated with TGT-PRIMAAGE. Treatment with TGT-PRIMAAGE showed significant reduction (P < 0.05) in the number of senescent cells. Significant reduction (P < 0.05) of malondialdehyde was also seen in cells treated with TGT-PRIMAAGE. The p53 protein level was reduced in TGT-PRIMAAGE-treated cells, which indicates its potential in protecting the cells from oxidative stress induced by H2O2.

引用

页码：33 / 44

页数：12

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