Purification and characterization of a lectin from Xanthomonas campestris NCIM 5028

被引：2

作者：

Joshi, B ^{[1
]}

Khire, JM ^{[1
]}

SivaRaman, H ^{[1
]}

Khan, MI ^{[1
]}

机构：

[1] NATL CHEM LAB,DIV BIOCHEM SCI,PUNE 411008,MAHARASHTRA,INDIA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1996年 / 42卷 / 06期

关键词：

Xanthomonas campestris; lectin; purification;

D O I：

10.1139/m96-082

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A lectin was isolated from culture filtrates of Xanthomonas campestris NCIM 5028, by a simple procedure of hydrophobic chromatography on phenyl-Sepharose after ammonium sulphate precipitation. The lectin was a heterodimer, with subunit molecular masses of 30 000 and 28 000. Gel filtration on S-300 column, calibrated with markers, showed its molecular mass to be approximately 70 000. Its isoelectric point was 7.2. The agglutination of the rabbit erythrocytes by the lectin was inhibited by fetuin glycopeptides and host plant (Brassica oleracea) extracts.

引用

页码：609 / 612

页数：4

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