Purification and characterization of a lectin from Xanthomonas campestris NCIM 5028

被引：2

作者：

Joshi, B ^{[1
]}

Khire, JM ^{[1
]}

SivaRaman, H ^{[1
]}

Khan, MI ^{[1
]}

机构：

[1] NATL CHEM LAB,DIV BIOCHEM SCI,PUNE 411008,MAHARASHTRA,INDIA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1996年 / 42卷 / 06期

关键词：

Xanthomonas campestris; lectin; purification;

D O I：

10.1139/m96-082

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A lectin was isolated from culture filtrates of Xanthomonas campestris NCIM 5028, by a simple procedure of hydrophobic chromatography on phenyl-Sepharose after ammonium sulphate precipitation. The lectin was a heterodimer, with subunit molecular masses of 30 000 and 28 000. Gel filtration on S-300 column, calibrated with markers, showed its molecular mass to be approximately 70 000. Its isoelectric point was 7.2. The agglutination of the rabbit erythrocytes by the lectin was inhibited by fetuin glycopeptides and host plant (Brassica oleracea) extracts.

引用

页码：609 / 612

页数：4

共 20 条

[1] MOLECULAR-GENETICS OF EXTRACELLULAR POLYSACCHARIDE BIOSYNTHESIS IN VASCULAR PHYTOPATHOGENIC BACTERIA [J].

COPLIN, DL ;

COOK, D .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1990, 3 (05) :271-279

[2] GENETIC-EVIDENCE THAT EXTRACELLULAR POLYSACCHARIDE IS A VIRULENCE FACTOR OF PSEUDOMONAS-SOLANACEARUM [J].

DENNY, TP ;

BAEK, SR .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1991, 4 (02) :198-206

[3]

DEPIERREUX C, 1991, Glycobiology, V1, P643, DOI 10.1093/glycob/1.6.643

[4]

DESTEVENS G, 1956, METHOD ENZYMOL, V3, P31

[5] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

[6] CELL CELL RECOGNITION OF HOST SURFACES BY PATHOGENS - THE ADSORPTION OF MAIZE (ZEA-MAYS) ROOT MUCILAGE BY SURFACES OF PATHOGENIC FUNGI [J].

GOULD, J ;

NORTHCOTE, DH .

BIOCHEMICAL JOURNAL, 1986, 233 (02) :395-405

[7]

HO HC, 1990, J CELL BIOL, V111, P1631

[8] A NEWLY ISOLATED LECTIN FROM THE PLANT-PATHOGENIC FUNGUS SCLEROTIUM-ROLFSII - PURIFICATION, CHARACTERIZATION AND ROLE IN MYCOPARASITISM [J].

INBAR, J ;

CHET, I .

MICROBIOLOGY-SGM, 1994, 140 :651-657

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

LIS H, 1978, J BIOL CHEM, V253, P3468

← 1 2 →