Oriented macroporous polyacrylamide gels

被引:4
作者
Charlionet, R [1 ]
MachourMerlet, N [1 ]
Leclerc, S [1 ]
Malandain, JJ [1 ]
机构
[1] UNIV ROUEN,CNRS UMR6634,MONT ST AIGNAN,FRANCE
关键词
macroporosity; polyacrylamide gel; oriented gel; scanning electron microscopy;
D O I
10.1002/elps.1150180718
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Macroporous gels with huge cavities and partition walls result from controlled microsyneresis during gelation. In this report we show that the microsyneresis process can be further controlled: it is possible to orient the partition walls of macropores by the use of an electric field throughout polymerization. Our oriented macroporous polyacrylamide gels offer a specific structure: a set of parallel channels of about 2 mu m inner diameter, with polyacrylamide walls including acidic groups linked to the aggregated matter.
引用
收藏
页码:1133 / 1135
页数:3
相关论文
共 8 条
[1]   LARGE-SCALE MICROSEGREGATION IN POLYACRYLAMIDE GELS (SPINODAL GELS) [J].
ASNAGHI, D ;
GIGLIO, M ;
BOSSI, A ;
RIGHETTI, PG .
JOURNAL OF CHEMICAL PHYSICS, 1995, 102 (24) :9736-9742
[2]  
CHARLIONET R, 1993, ELECTROPHORESIS, V17, P58
[3]  
DEGENNE PG, 1993, SCALING CONCEPTS POL
[4]   ON THE KINETICS OF MONOMER INCORPORATION INTO POLYACRYLAMIDE GELS, AS INVESTIGATED BY CAPILLARY ZONE ELECTROPHORESIS [J].
RIGHETTI, PG ;
CAGLIO, S .
ELECTROPHORESIS, 1993, 14 (07) :573-582
[5]   LATERALLY AGGREGATED POLYACRYLAMIDE GELS FOR ELECTROPHORESIS [J].
RIGHETTI, PG ;
CAGLIO, S ;
SARACCHI, M ;
QUARONI, S .
ELECTROPHORESIS, 1992, 13 (9-10) :587-595
[6]   Protein electrophoresis in polyacrylamide gels with templated pores [J].
Rill, RL ;
Locke, BR ;
Liu, YJ ;
Dharia, J ;
VanWinkle, D .
ELECTROPHORESIS, 1996, 17 (08) :1304-1312
[8]   CONVENTIONAL ISOELECTRIC-FOCUSING AND IMMOBILIZED PH GRADIENTS IN MACROPOROUS POLYACRYLAMIDE GELS [J].
WENISCH, E ;
DEBESI, P ;
RIGHETTI, PG .
ELECTROPHORESIS, 1993, 14 (07) :583-590