Expression of LOXs and MMP-1, 2, 3 by ACL Fibroblasts and Synoviocytes Impact of Coculture and TNF-α

被引：9

作者：

Wang, Chunli ^{[1
]}

Chi, Qingjia ^{[2
]}

Xu, Chunming ^{[1
]}

Xu, Kang ^{[1
]}

Zhang, Yanjun ^{[4
]}

Liu, Yan ^{[1
]}

Yang, Li ^{[1
]}

Sung, K. L. Paul ^{[1
,3
]}

机构：

[1] Chongqing Univ, Bioengn Coll, Project Lab Biomech & Tissue Repair 111, Chongqing 400044, Peoples R China

[2] Wuhan Univ Technol, Dept Mech & Engn Struct, Hubei Key Lab Theory & Applicat Adv Mat Mech, Wuhan, Hubei, Peoples R China

[3] Univ Calif San Diego, Dept Bioengn & Orthopaed, La Jolla, CA 92093 USA

[4] Hunan Univ Sci & Technol, Dept Life Sci, Xiangtan, Hunan, Peoples R China

来源：

JOURNAL OF KNEE SURGERY | 2019年 / 32卷 / 04期

关键词：

ACL; synoviocytes; MMPs; LOXs; coculture; MEDIAL COLLATERAL LIGAMENT; ANTERIOR CRUCIATE LIGAMENT; NECROSIS-FACTOR-ALPHA; LYSYL OXIDASE FAMILY; MATRIX METALLOPROTEINASES; EXTRACELLULAR-MATRIX; MECHANICAL STRETCH; IN-VITRO; CELLS; ADHESION;

D O I：

10.1055/s-0038-1641592

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

This study aims to confirm the effects of synoviocytes (SCs) on regulating lysyl oxidases (LOXs) and matrix metalloproteinase (MMP)-1, 2, 3 in the normal and injured anterior cruciate ligament (ACL) fibroblasts response to tumor necrosis factor-(TNF-alpha). The gene and protein expression levels of LOXs and MMP-1, 2, 3 in SCs cocultured ACL fibroblasts (ACLfs) induced by TNF-alpha and mechanical injury were analyzed by real-time polymerase chain reaction (PCR) and western bolting; the MMP-2 activity were analyzed by zymography. The results exhibited that TNF-alpha alone slightly downregulated the expressions of LOXs and upregulated the expression of MMP-1, 2, 3 in both normal and injured ACL fibroblasts. The decrease of LOXs and increase of MMP-1, 2, 3 in ACLfs response to TNF-alpha were further promoted by coculture. Taken together, these results show for the first time that the crosstalk between ACLfs and SCs could modulate the LOXs and MMP-1, 2, 3 synthesis in ACLfs in the presence of TNF-alpha. Accumulation of MMPs in the isolated fluid-containing space not only disrupts the balance of ACL healing, but also increases cartilage degradation and accelerates osteoarthritis (OA) in injured joint. Based on this mechanism, targeting inhibition of MMPs could provide a promising therapeutic strategy for acute ligament injury.

引用

页码：352 / 360

页数：9

共 26 条

[1] PATHOPHYSIOLOGY OF SOFT-TISSUE REPAIR [J].

BARLOW, Y ;

WILLOUGHBY, J .

BRITISH MEDICAL BULLETIN, 1992, 48 (03) :698-711

[2] Influences of Tumor Necrosis Factor-α on Lysyl Oxidases and Matrix Metalloproteinases of Injured Anterior Cruciate Ligament and Medial Collateral Ligament Fibroblasts [J].

Cai, Linyi ;

An, Shu ;

Liao, Jinfeng ;

Yang, Wenbin ;

Zhou, Xuedong ;

Sung, K. L. Paul ;

Xie, Jing .

JOURNAL OF KNEE SURGERY, 2017, 30 (01) :78-87

[3] Gene profiling of the rat medial collateral ligament during early healing using microarray analysis [J].

Chamberlain, Connie S. ;

Brounts, Sabrina H. ;

Sterken, David G. ;

Rolnick, Kevin I. ;

Baer, Geoffrey S. ;

Vanderby, Ray .

JOURNAL OF APPLIED PHYSIOLOGY, 2011, 111 (02) :552-565

[4] In vivo measurement of ligament/tendon strains and forces:: A review [J].

Fleming, BC ;

Beynnon, BD .

ANNALS OF BIOMEDICAL ENGINEERING, 2004, 32 (03) :318-328

[5] Tumor necrosis factor alpha inhibits type I collagen synthesis through repressive CCAAT/enhancer-binding proteins [J].

Greenwel, P ;

Tanaka, S ;

Penkov, D ;

Zhang, W ;

Olive, M ;

Moll, J ;

Vinson, C ;

Di Liberto, M ;

Ramirez, F .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (03) :912-918

[6] Cathepsin K is a critical protease in synovial fibroblast-mediated collagen degradation [J].

Hou, WS ;

Li, ZQ ;

Gordon, RE ;

Chan, K ;

Klein, MJ ;

Levy, R ;

Keysser, M ;

Keyszer, G ;

Brömme, D .

AMERICAN JOURNAL OF PATHOLOGY, 2001, 159 (06) :2167-2177

[7] Intraarticular inflammatory cytokines in acute anterior cruciate ligament injured knee [J].

Irie, K ;

Uchiyama, E ;

Iwaso, H .

KNEE, 2003, 10 (01) :93-96

[8] Lysyl oxidase: Properties, specificity, and biological roles inside and outside of the cell [J].

Kagan, HM ;

Li, WD .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2003, 88 (04) :660-672

[9] Basic science of anterior cruciate ligament injury and repair [J].

Kiapour, A. M. ;

Murray, M. M. .

BONE & JOINT RESEARCH, 2014, 3 (02) :20-31

[10] The long-term consequence of anterior cruciate ligament and meniscus injuries - Osteoarthritis [J].

Lohmander, L. Stefan ;

Englund, P. Martin ;

Dahl, Ludvig L. ;

Roos, Ewa M. .

AMERICAN JOURNAL OF SPORTS MEDICINE, 2007, 35 (10) :1756-1769

← 1 2 3 →