Flow Cytometry Analysis with a New FITC-Conjugated Monoclonal Antibody-3E12 for HLA-B*57:01 Rapid Screening in Prevention of Abacavir Hypersensitivity in HIV-1-Infected Patients

被引：6

作者：

Rodriguez-Sainz, C. ^{[1
]}

Valor, L. ^{[1
]}

Hernandez, D. C. ^{[1
]}

Gil, J. ^{[1
]}

Carbone, J. ^{[1
]}

Pascual-Bernaldez, M. ^{[2
]}

Rodriguez-Alcantara, F. ^{[3
]}

Martinez, I. ^{[1
]}

Vicario, J. L. ^{[4
]}

Mallal, S. ^{[5
,6
]}

Fernandez-Cruz, E. ^{[1
]}

机构：

[1] Univ Complutense Madrid, Dept Microbiol Inmunol 1, Hosp Gen Univ Gregorio Maranon, Serv Inmunol Clin, Madrid, Spain

[2] GlaxoSmithKline, Dept Med, Madrid, Spain

[3] ViiV Healthcare, Madrid, Spain

[4] Ctr Transfus Comunidad Madrid, Dept Histocompatibilidad, Madrid, Spain

[5] Royal Perth Hosp, Inst Immunol & Infect Dis, Perth, WA, Australia

[6] Murdoch Univ, Murdoch, WA 6150, Australia

来源：

HIV CLINICAL TRIALS | 2013年 / 14卷 / 04期

关键词：

abacavir; DNA-PCR technique; drug hypersensitivity; flow cytometry; HLA-B*57:01; ADULT HIV-INFECTION; ANTIRETROVIRAL TREATMENT; REVERSE-TRANSCRIPTASE; RECOMMENDATIONS; ASSOCIATION;

D O I：

10.1310/hct1404-160

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: Rapid screening for the detection of HLA-B*57:01 in the prevention of abacavir hypersensitivity in HIV-1-infected patients is a hallmark for clinical services. Objective: The aim of this work was to analyze the utility of flow cytometry with a new FITC-conjugated B-17 monoclonal antibody (mAb3E12) for HLA-B*57:01 screening in a Spanish cohort of 577 HIV-1+ individuals. Methods: Cryopreserved peripheral blood mononuclear cell samples from HIV-1+ individuals were analyzed by flow cytometry with the mAb 3E12 that recognizes both HLA-B*57 and HLA-B*58 alleles (members of the group specificity, HLA-B17). Patients' DNA samples had been previously typed for HLA-B*57:01 with PCR-SSO or PCR-SSP and additional DNA sequencing (EPI Study). The results obtained by flow cytometry were compared with the results obtained by the DNA-PCR techniques. Results: By flow cytometry, 46 samples (7.97%) were positive for HLA-B17, 530 (91.86%) were negative, and 1 (0.17%) was undetermined. All samples found negative by flow cytometry were negative for HLA-B*57:01 by DNA-PCR. Of the HLA-B17 positive samples, 31(67.4%) were positive for HLA-B*57:01, 2(3.25%) were positive for HLA-B*57:03, 11 (26.1%) were positive for HLA-B*58, and 2 (3.25%) were negative for both HLA-B*57 and HLA-B*58 antigens. The undetermined sample was negative for HLA-B*57 and HLA-B*58 alleles by DNA-PCR. Conclusions: This study shows that flow cytometry with mAb3E12 is a highly sensitive method (no false negatives) to implement prior to DNA-PCR analysis for rapid screening of HLA-B*57:01. Additional confirmation by molecular HLA typing method would be required in less than 10% of the cohort of HIV-1-infected individuals.

引用

页码：160 / 164

页数：5