E. adenophorum Induces Cell Cycle and Apoptosis of Renal Cells through Mitochondrial Pathway and Caspase Activation in Saanen Goat

被引：19

作者：

He, Yajun ^{[1
]}

Chen, Weihong ^{[1
]}

Hu, Yanchun ^{[1
]}

Luo, Biao ^{[1
]}

Wu, Lei ^{[1
]}

Qiao, Yan ^{[1
]}

Mo, Quan ^{[1
]}

Xu, Ruiguang ^{[1
]}

Zhou, Yancheng ^{[1
]}

Ren, Zhihua ^{[1
]}

Zuo, Zhicai ^{[1
]}

Deng, Junliang ^{[1
]}

Peng, Guangneng ^{[1
]}

He, Wei ^{[2
]}

Wei, Yahui ^{[2
]}

机构：

[1] Sichuan Agr Univ, Key Lab Anim Dis & Human Hlth Sichuan Prov, Coll Vet Med, Wenjiang, Sichuan, Peoples R China

[2] Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Sch Life Sci, Xian, Peoples R China

来源：

PLOS ONE | 2015年 / 10卷 / 09期

关键词：

EUPATORIUM-ADENOPHORUM; CYTOCHROME-C; BCL-2; P53; HEPATOTOXICITY; CPP32; 9-OXO-10,11-DEHYDROAGERAPHORONE; SARCOPTES; EXTRACTS; CLEAVAGE;

D O I：

10.1371/journal.pone.0138504

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cytotoxicity effects of E. adenophorum on cell cycle and apoptosis of renal cells in Saanen goat was evaluated by TUNEL, DAPI, AO/EB staining, DNA fragmentation assay, Caspase activity, Western-blot, qRT-PCR and flow cytometry analysis. 16 saanen goats randomly divided into four groups were fed on 0%, 40%, 60% and 80% E. adenophorum diets. The Results showed that E. adenophorum induced typical apoptotic features of renal cells. E. adenophorum significantly suppressed renal cells viability, caused cell cycle activity arrest and induced typical apoptotic features in a dose-dependent manner. However, the protein levels of Fas/FasL, Bid and caspase-8 did not appear significant changes in the process of E. adenophorum-induced apoptosis. Moreover, E. adenophorum administration slightly decreased Bcl-2 expression, promoted Bax translocation to mitochondria, triggered the release of Cyt c from mitochondria into cytosol and activated caspase-9, -3, and cleaved PARP. The mitochondrial p53 translocation was significantly activated, accompanied by a significant increase in the loss of Delta psi m, Cyt c release and caspase-9 activation. Above all, these data suggest that E. adenophorum induces renal cells apoptosis via the activation of mitochondria-mediated apoptosis pathway in renal cells. These findings may provide new insights to understand the mechanisms involved in E. adenophorum-caused cytotoxicity of renal cells.

引用

页数：16

共 49 条

[1] Hepatotoxicity and cholestasis in rats induced by the sesquiterpene, 9-oxo-10,11-dehydroageraphorone, isolated from Eupatorium adenophorum [J].