Prostaglandin E2 reinforces the activation of Ras signal pathway in lung adenocarcinoma cells via EP3

Prostaglandin E-2 (PGE(2))-dependent effects on various cell responses are regulated by respective PGE(2) receptors (EP1, EP2, EP3, EP4) expressing in target cells. Alveolar type H cell (a main progenitor cell of lung adenocarcinoma) expressed only EP4, while human lung adenocarcinoma cells (A549) expressed EP3 as well as EP4. An antagonistic effect of EP3 against EP4 through the modulation of cyclic AATP level is required for PGE(2)-mediated activation of Ras signal pathway in A549 cells. These results suggest that the expression of EP3 may be a critical factor for the PGE(2)-mediated activation of Ras signal pathway in A549 cells. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.