Shiga toxin-producing Escherichia coli O157 in feedlot cattle and Norwegian rats from a large-scale farm

被引：59
作者：
Cízek, A
Alexa, P
Literák, I
Hamrík, J
Novák, P
Smola, J
机构：
[1] Univ Vet & Pharmaceut Sci, Fac Vet Med, Brno 61242, Czech Republic
[2] Vet Res Inst, CS-62132 Brno, Czech Republic
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 28卷 / 06期
关键词：
D O I：
10.1046/j.1365-2672.1999.00549.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A total of 365 faecal samples from different categories of cattle, 12 samples of untreated slurry, 50 samples of fresh droppings of fetal domestic pigeons, 20 samples of fresh droppings of domestic sparrows and stool samples of 19 synanthropic rodents were examined for the presence of Escherichia coli by broth enrichment culture and a subsequent immunomagnetic separation. Escherichia coli O157 was found in 72 (20%) bovine samples, sis (500%) samples of untreated slurry and four (40%) of 10 rats (Rattus norvegicus). Significant differences were found in the E. coli O157 shedding frequency between different age categories of bulls. Genes stx2 and eaeA were detected in all isolates, and the stx1 gene in all but 10 isolates.
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页码：435 / 439
页数：5
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