Multichannel diffuse optical Raman tomography for bone characterization in vivo: a phantom study

被引：38

作者：

Demers, Jennifer-Lynn H. ^{[1
]}

Davis, Scott C. ^{[1
]}

Pogue, Brian W. ^{[1
]}

Morris, Michael D. ^{[2
]}

机构：

[1] Dartmouth Coll, Thayer Sch Engn, Hanover, NH 03755 USA

[2] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2012年 / 3卷 / 09期

关键词：

SPECTROSCOPY; TISSUE; MINERALIZATION; FLUORESCENCE; ABSORBER; COLLAGEN;

D O I：

10.1364/BOE.3.002299

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Raman spectroscopy is used to gather information on the mineral and organic components of bone tissue to analyze their composition. By measuring the Raman signal of bone through spatially offset Raman spectroscopy the health of the bone can be determined. We've customized a system with 8 collection channels that consist of individual fibers, which are coupled to separate spectrometers and cooled CCDs. This parallel detection system was used to scan gelatin phantoms with Teflon inclusions of two sizes. Raman signals were decoupled from the autofluorescence background using channel specific polynomial fitting. Images with high contrast to background ratios of Raman yield and accurate spatial resolution were recovered using a model-based diffuse tomography approach. (c) 2012 Optical Society of America

引用

页码：2299 / 2305

页数：7

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