Nitric oxide-eluting nanocomposite for cardiovascular implants

被引：24

作者：

de Mel, Achala ^{[1
]}

Naghavi, Noora ^{[1
]}

Cousins, Brian G. ^{[1
]}

Clatworthy, Innes ^{[2
]}

Hamilton, George ^{[3
]}

Darbyshire, Arnold ^{[1
]}

Seifalian, Alexander M. ^{[1
,3
]}

机构：

[1] UCL, Ctr Nanotechnol & Regenerat Med, London, England

[2] Royal Free Hosp, Electron Microscopy Unit, London NW3 2QG, England

[3] Royal Free Hampstead NHS Trust Hosp, London, England

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2014年 / 25卷 / 03期

关键词：

IN-SITU ENDOTHELIALIZATION; S-NITROSOTHIOLS; CELL-GROWTH; REAL-TIME; MECHANISM; HEMOCOMPATIBILITY; BIOMATERIALS; GENERATION; GRAFTS; BLOOD;

D O I：

10.1007/s10856-013-5103-2

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Cardiovascular implants must resist thrombosis and intimal hyperplasia, but they are prone to such patency limiting conditions during graft implantation and prior to endothelialisation. Nitric oxide (NO) released from the endothelium has a complex protective role in the cardiovascular system, and this study has addressed: (1) in situ NO release profiles from S-nitrosothiols ((S-Nitroso-N-acetylpenicillamine (SNAP) and (S-Nitrosoglutathione (GSNO)) incorporated into polyhedral oligomeric silsesquioxanepoly(carbonate-urea)urethane (POSS-PCU) coronary artery bypass grafts (CABG) in a physiological pulsatile flow, and (2) the determination of their interaction with endothelial progenitor cells (EPCs), smooth muscle cells, platelets, whole blood kinetics. It was found that 1, 2, and 3 wt% SNAP/GSNO incorporated into POSS-PCU-CABG successfully eluted NO, but optimal elution was evident with 2 %-SNAP-POSS-PCU. NO release determined under static conditions using the Griess assay, and in situ measurements under pulsatile flow using amperometric probe was found to differ, thus confirming the significance of monitoring NO-elution under haemodynamic conditions. 2 %-SNAP-POSS-PCU demonstrated anti-thrombogenic kinetics through thromboelastography measurements, while metabolic activity using Alamar Blue (TM) assay and scanning electron microscopy demonstrated greater adhesion of EPCs and reduced adhesion of platelets.

引用

页码：917 / 929

页数：13

共 41 条

[1] Small calibre polyhedral oligomeric silsesquioxane nanocomposite cardiovascular grafts: Influence of porosity on the structure, haemocompatibility and mechanical properties [J].

Ahmed, Maqsood ;

Ghanbari, Hossein ;

Cousins, Brian G. ;

Hamilton, George ;

Seifalian, Alexander M. .

ACTA BIOMATERIALIA, 2011, 7 (11) :3857-3867

[2] The determination of S-nitrosothiols in biological samples-Procedures, problems and precautions [J].

Bramanti, E. ;

Angeli, V. ;

Paolicchi, A. ;

Pompella, A. .

LIFE SCIENCES, 2011, 88 (3-4) :126-129

[3] The reaction mechanism of nitrosothiols with copper(I) [J].

Burg, A ;

Cohen, H ;

Meyerstein, D .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2000, 5 (02) :213-217

[4] Development of a new lacrimal drainage conduit using POSS nanocomposite [J].

Chaloupka, Karla ;

Motwani, Meghna ;

Seifalian, Alexander Marcus .

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2011, 58 (05) :363-370

[5] Degradable Nitric Oxide-Releasing Biomaterials via Post-Polymerization Functionalization of Cross-Linked Polyesters [J].

Coneski, Peter N. ;

Rao, Kavitha S. ;

Schoenfisch, Mark H. .

BIOMACROMOLECULES, 2010, 11 (11) :3208-3215

[6]

Davies IR, 2008, METHOD ENZYMOL, P1

[7] Biofunctionalization of Biomaterials for Accelerated in Situ Endothelialization: A Review [J].

de Mel, Achala ;

Jell, Gavin ;

Stevens, Molly M. ;

Seifalian, Alexander M. .

BIOMACROMOLECULES, 2008, 9 (11) :2969-2979

[8] Nitric Oxide: A Guardian for Vascular Grafts? [J].

de Mel, Achala ;

Murad, Ferid ;

Seifalian, Alexander M. .

CHEMICAL REVIEWS, 2011, 111 (09) :5742-5767

[9] In situ endothelialisation potential of a biofunctionalised nanocomposite biomaterial-based small diameter bypass graft [J].

de Mel, Achala ;

Punshon, Geoffrey ;

Ramesh, Bala ;

Sarkar, Sandip ;

Darbyshire, Arnold ;

Hamilton, George ;

Seifalian, Alexander M. .

BIO-MEDICAL MATERIALS AND ENGINEERING, 2009, 19 (4-5) :317-331

[10] Generation of nitric oxide from S-nitrosothiols using protein-bound Cu2+ sources [J].

Dicks, AP ;

Williams, DLH .

CHEMISTRY & BIOLOGY, 1996, 3 (08) :655-659

← 1 2 3 4 5 →