Mariniflexile jejuense sp nov., isolated from the junction between seawater and a freshwater spring, and emended description of the genus Mariniflexile

A Gram-negative, non-spore-forming, non-flagellated, motile-by-gliding rod, designated SSK2-3(T), was isolated from the junction between seawater and a freshwater spring at Jeju island, South Korea. Strain SSK2-3(T) grew optimally at 25-30 degrees C, at pH 7.0-7.5 and in the presence of 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SSK2-3(T) clustered with type strains of species of the genus Mariniflexile, with which it exhibited 97.2-97.8% 16S rRNA gene sequence similarity. Sequence similarity between the isolate and the other strains used in the phylogenetic analysis was <95.6%. Strain SSK2-3(T) contained MK-6 as the predominant menaquinone and iso-C-15:0, iso-C-15:1 G and C-15:0 as the major fatty acids. The major polar lipids of strain SSK2-3(T) were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-3(T) was 32.4 mol%. DNA-DNA relatedness between the isolate and Mariniflexile gromovii KCTC 12570(T), Mariniflexile fucanivorans DSM 18792(T) and Mariniflexile aquimaris HWR-17(T) was 19, 15 and 20 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SSK2-3(T) is separate from other members of the genus Mariniflexile. On the basis of the data presented, strain SSK2-3(T) is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile jejuense sp. nov. is proposed. The type strain is SSK2-3(T) (=KCTC 23958(T) =CCUG 62414(T)). An emended description of the genus Mariniflexile is given.