Kinetics of 1,2-Dichloroethane and 1,2-Dibromoethane Biodegradation in Anaerobic Enrichment Cultures

被引:31
作者
Yu, Rong [1 ]
Peethambaram, Hari S. [1 ]
Falta, Ronald W. [1 ]
Verce, Matthew F. [2 ]
Henderson, James K. [3 ]
Bagwell, Christopher E. [4 ]
Brigmon, Robin L. [4 ]
Freedman, David L. [1 ]
机构
[1] Clemson Univ, Dept Environm Engn & Earth Sci, Clemson, SC 29634 USA
[2] Weiss Associates, Emeryville, CA USA
[3] DuPont Corp, Remediat Grp, Charlotte, NC USA
[4] Savannah River Natl Lab, Aiken, SC USA
关键词
DICHLOROELIMINANS STRAIN DCA1; VINYL-CHLORIDE; REDUCTIVE DECHLORINATION; CHLORINATED ETHENES; ETHYLENE DIBROMIDE; GROUNDWATER; GROWTH; DEHALOGENASE; BACTERIUM; DETOXIFICATION;
D O I
10.1128/AEM.02163-12
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
1,2-Dichloroethane (1,2-DCA) and 1,2-dibromoethane (ethylene dibromide [EDB]) contaminate groundwater at many hazardous waste sites. The objectives of this study were to measure yields, maximum specific growth rates ((mu) over cap), and half-saturation coefficients (K-S) in enrichment cultures that use 1,2-DCA and EDB as terminal electron acceptors and lactate as the electron donor and to evaluate if the presence of EDB has an effect on the kinetics of 1,2-DCA dehalogenation and vice versa. Biodegradation was evaluated at the high concentrations found at some industrial sites (>10 mg/liter) and at lower concentrations found at former leaded-gasoline sites (1.9 to 3.7 mg/liter). At higher concentrations, the Dehalococcoides yield was 1 order of magnitude higher when bacteria were grown with 1,2-DCA than when they were grown with EDB, while (mu) over cap 's were similar for the two compounds, ranging from 0.19 to 0.52 day(-1) with 1,2-DCA to 0.28 to 0.36 day(-1) for EDB. K-S was larger for 1,2-DCA (15 to 25 mg/liter) than for EDB (1.8 to 3.7 mg/liter). In treatments that received both compounds, EDB was always consumed first and adversely impacted the kinetics of 1,2-DCA utilization. Furthermore, 1,2-DCA dechlorination was interrupted by the addition of EDB at a concentration 100 times lower than that of the remaining 1,2-DCA; use of 1,2-DCA did not resume until the EDB level decreased close to its maximum contaminant level (MCL). In lower-concentration experiments, the preferential consumption of EDB over 1,2-DCA was confirmed; both compounds were eventually dehalogenated to their respective MCLs (5 mu g/liter for 1,2-DCA, 0.05 mu g/liter for EDB). The enrichment culture grown with 1,2-DCA has the advantage of a more rapid transition to 1,2-DCA after EDB is consumed.
引用
收藏
页码:1359 / 1367
页数:9
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